Beyond bacteria: A study of the enteric microbial consortium in extremely low birth weight infants

Mariam Susan LaTuga, Joseph Christopher Ellis, Charles Michael Cotton, Ronald N. Goldberg, James L. Wynn, Robert B. Jackson, Patrick C. Seed

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Extremely low birth weight (ELBW) infants have high morbidity and mortality, frequently due to invasive infections from bacteria, fungi, and viruses. The microbial communities present in the gastrointestinal tracts of preterm infants may serve as a reservoir for invasive organisms and remain poorly characterized. We used deep pyrosequencing to examine the gut-associated microbiome of 11 ELBW infants in the first postnatal month, with a first time determination of the eukaryote microbiota such as fungi and nematodes, including bacteria and viruses that have not been previously described. Among the fungi observed, Candida sp. and Clavispora sp. dominated the sequences, but a range of environmental molds were also observed. Surprisingly, seventy-one percent of the infant fecal samples tested contained ribosomal sequences corresponding to the parasitic organism Trichinella. Ribosomal DNA sequences for the roundworm symbiont Xenorhabdus accompanied these sequences in the infant with the greatest proportion of Trichinella sequences. When examining ribosomal DNA sequences in aggregate, Enterobacteriales, Pseudomonas, Staphylococcus, and Enterococcus were the most abundant bacterial taxa in a low diversity bacterial community (mean Shannon-Weaver Index of 1.02±0.69), with relatively little change within individual infants through time. To supplement the ribosomal sequence data, shotgun sequencing was performed on DNA from multiple displacement amplification (MDA) of total fecal genomic DNA from two infants. In addition to the organisms mentioned previously, the metagenome also revealed sequences for gram positive and gram negative bacteriophages, as well as human adenovirus C. Together, these data reveal surprising eukaryotic and viral microbial diversity in ELBW enteric microbiota dominated bytypes of bacteria known to cause invasive disease in these infants.

Original languageEnglish (US)
Article numbere27858
JournalPLoS One
Volume6
Issue number12
DOIs
StatePublished - Dec 8 2011

Fingerprint

Microbial Consortia
Extremely Low Birth Weight Infant
low birth weight
Fungi
Bacteria
Trichinella
DNA sequences
Ribosomal DNA
Viruses
ribosomal DNA
fungi
bacteria
organisms
Human adenovirus C
Enterobacteriales
Clavispora
Xenorhabdus
Nematoda
Microbiota
nucleotide sequences

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

LaTuga, M. S., Ellis, J. C., Cotton, C. M., Goldberg, R. N., Wynn, J. L., Jackson, R. B., & Seed, P. C. (2011). Beyond bacteria: A study of the enteric microbial consortium in extremely low birth weight infants. PLoS One, 6(12), [e27858]. https://doi.org/10.1371/journal.pone.0027858
LaTuga, Mariam Susan ; Ellis, Joseph Christopher ; Cotton, Charles Michael ; Goldberg, Ronald N. ; Wynn, James L. ; Jackson, Robert B. ; Seed, Patrick C. / Beyond bacteria : A study of the enteric microbial consortium in extremely low birth weight infants. In: PLoS One. 2011 ; Vol. 6, No. 12.
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Beyond bacteria : A study of the enteric microbial consortium in extremely low birth weight infants. / LaTuga, Mariam Susan; Ellis, Joseph Christopher; Cotton, Charles Michael; Goldberg, Ronald N.; Wynn, James L.; Jackson, Robert B.; Seed, Patrick C.

In: PLoS One, Vol. 6, No. 12, e27858, 08.12.2011.

Research output: Contribution to journalArticle

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LaTuga MS, Ellis JC, Cotton CM, Goldberg RN, Wynn JL, Jackson RB et al. Beyond bacteria: A study of the enteric microbial consortium in extremely low birth weight infants. PLoS One. 2011 Dec 8;6(12). e27858. https://doi.org/10.1371/journal.pone.0027858