TY - JOUR
T1 - Bilateral Arteriovenous Shunts as a Method for Evaluating Tissue-Engineered Vascular Grafts in Large Animal Models
AU - Ong, Chin Siang
AU - Fukunishi, Takuma
AU - Liu, Rui Han
AU - Nelson, Kevin
AU - Zhang, Huaitao
AU - Wieczorek, Elizabeth
AU - Palmieri, McKenna
AU - Ueyama, Yukie
AU - Ferris, Erin
AU - Geist, Gail E.
AU - Youngblood, Brad
AU - Johnson, Jed
AU - Hibino, Narutoshi
N1 - Publisher Copyright:
© Copyright 2017, Mary Ann Liebert, Inc. 2017.
PY - 2017/11
Y1 - 2017/11
N2 - There remains a need for large animal models to evaluate tissue-engineered vascular grafts (TEVGs) under arterial pressure to provide preclinical data for future potential human clinical trials. We present a comprehensive method for the interrogation of TEVGs, using an ovine bilateral arteriovenous (AV) shunt implantation model. Our results demonstrate that this method can be performed safely without complications, specifically acute heart failure, steal syndrome, and hypoxic brain injury, and it is a viable experimental paradigm. Our method allows for a non-invasive evaluation of TEVGs in terms of graft flow, graft diameter, and graft patency, while also allowing for graft needle puncture under ultrasound guidance. In addition, traditional pathological analysis, histology, and immunohistochemistry may be performed with the contralateral side providing paired control data to eliminate inter-subject variability while reducing the total number of animals. Further, we present a review of existing literature of preclinical evaluation of TEVGs in large animal models as AV conduits.
AB - There remains a need for large animal models to evaluate tissue-engineered vascular grafts (TEVGs) under arterial pressure to provide preclinical data for future potential human clinical trials. We present a comprehensive method for the interrogation of TEVGs, using an ovine bilateral arteriovenous (AV) shunt implantation model. Our results demonstrate that this method can be performed safely without complications, specifically acute heart failure, steal syndrome, and hypoxic brain injury, and it is a viable experimental paradigm. Our method allows for a non-invasive evaluation of TEVGs in terms of graft flow, graft diameter, and graft patency, while also allowing for graft needle puncture under ultrasound guidance. In addition, traditional pathological analysis, histology, and immunohistochemistry may be performed with the contralateral side providing paired control data to eliminate inter-subject variability while reducing the total number of animals. Further, we present a review of existing literature of preclinical evaluation of TEVGs in large animal models as AV conduits.
KW - arteriovenous shunts
KW - electrospinning
KW - large animal models
KW - nanofibers
KW - tissue-engineered vascular grafts
KW - vascular surgery
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U2 - 10.1089/ten.tec.2017.0217
DO - 10.1089/ten.tec.2017.0217
M3 - Article
C2 - 28741438
AN - SCOPUS:85033790099
SN - 1937-3384
VL - 23
SP - 728
EP - 735
JO - Tissue Engineering - Part C: Methods
JF - Tissue Engineering - Part C: Methods
IS - 11
ER -