Binding of cytoskeletal proteins by monoclonal anti-DNA lupus autoantibodies

Janine André-Schwartz*, Syamal K. Datta, Yehuda Schoenfeld, David A. Isenberg, B. David Stollar, Robert S. Schwartz

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

133 Scopus citations

Abstract

Monoclonal anti-DNA antibodies produced by hybridomas derived from MRL- lpr lpr mice and human lupus patients were found to bind to the cytoskeleton of mink lung cells. When tested by indirect immunofluorescence, 17 29 human monoclonal anti-DNA antibodies reacted with the cytoskeleton; 4 of the 29 also produced antinuclear reactions with epithelial cells. The cytoskeletal staining was not inhibited by prior treatment of the cells with DNase, but it was completely blocked by prior incubation of the monoclonal antibodies with DNA and other nucleic acids. The ability of the polynucleotides to inhibit the cytoskeletal staining corresponded to their ability to bind to the antibodies in competitive immunoassays. An (Fab′)2 preparation of a monoclonal antibody bound to the cytoskeleton as well as the whole immunoglobulin. The effect of colcemid on the staining pattern, the blocking effect of a monoclonal antivimentin antibody, and results with nitrocellulose blots of cellular proteins indicated that the cytoskeletal protein to which the antibodies bound was vimentin.

Original languageEnglish (US)
Pages (from-to)261-271
Number of pages11
JournalClinical Immunology and Immunopathology
Volume31
Issue number2
DOIs
StatePublished - 1984

ASJC Scopus subject areas

  • Immunology and Allergy
  • Pathology and Forensic Medicine
  • Immunology

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