TY - JOUR
T1 - Binding of octa-plus porphyrazines to DNA
AU - Anderson, Máirín E.
AU - Barrett, Anthony G.M.
AU - Hoffman, Brian M.
N1 - Funding Information:
This work has been supported by the NSF (CHE 9727590).
PY - 2000/7/1
Y1 - 2000/7/1
N2 - We present a preliminary report of the interaction of octacationic N- methyl-pyridyl metalloporphyrazines, M = Cu(II) (Cupz+8) and Zn(II) (Znpz+8), with calf thymus DNA. These were monitored by electronic absorption spectroscopy, and in the case of Znpz+8, also by emission spectroscopy. These studies show that both the Cupz+8 and Znpz+8 interact strongly with DNA, and at sufficient concentrations induce a highly colored precipitate. The spectrum of Cupz+8 red-shifts as DNA is added, which is interpreted as indicative of π stacking of the charged macrocycle as it binds electrostatically to the outside of the DNA duplex. The spectrum of Znpz+8 does not shift, indicative of simple electrostatic surface binding. The absence of stacking may be related to the presence of an axial metal-ion ligand for Znpz+8, presumably 1120, while the metal ion of Cupz+8 is four-coordinate. (C) 2000 Published by Elsevier Science S.A.
AB - We present a preliminary report of the interaction of octacationic N- methyl-pyridyl metalloporphyrazines, M = Cu(II) (Cupz+8) and Zn(II) (Znpz+8), with calf thymus DNA. These were monitored by electronic absorption spectroscopy, and in the case of Znpz+8, also by emission spectroscopy. These studies show that both the Cupz+8 and Znpz+8 interact strongly with DNA, and at sufficient concentrations induce a highly colored precipitate. The spectrum of Cupz+8 red-shifts as DNA is added, which is interpreted as indicative of π stacking of the charged macrocycle as it binds electrostatically to the outside of the DNA duplex. The spectrum of Znpz+8 does not shift, indicative of simple electrostatic surface binding. The absence of stacking may be related to the presence of an axial metal-ion ligand for Znpz+8, presumably 1120, while the metal ion of Cupz+8 is four-coordinate. (C) 2000 Published by Elsevier Science S.A.
KW - Porphyrin
KW - Stacking
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U2 - 10.1016/S0162-0134(00)00084-2
DO - 10.1016/S0162-0134(00)00084-2
M3 - Article
C2 - 11001097
AN - SCOPUS:0034233379
SN - 0162-0134
VL - 80
SP - 257
EP - 260
JO - Journal of Inorganic Biochemistry
JF - Journal of Inorganic Biochemistry
IS - 3-4
ER -