Binding of ovalbumin to mouse spleen cells with and without carbodiimide

Richard Wetzig; Donald G. Hanson; Stephen D. Miller; Henry N. Claman

doi:10.1016/0022-1759(79)90201-1

Binding of ovalbumin to mouse spleen cells with and without carbodiimide

Richard Wetzig^*, Donald G. Hanson, Stephen D. Miller, Henry N. Claman

^*Corresponding author for this work

Microbiology-Immunology

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

We studied the binding of ovalbumin (OVA) to mouse spleen cells. In the presence or absence of carbodiimide (ECDI), uptake increased with greater cell numbers, increasing OVA concentration and increasing time up to 60 min. Between 60 and 210 μg OVA could be coupled with ECDI to 2 × 10⁸ cells. In the absence of ECDI, however, OVA uptake was still appreciable, but appeared to be of low avidity as it did not occur in the presence of coupeting fetal calf serum. These experiments provide a basis for the use of protein coupled to autologous cells in the induction of immunologic tolerance.

Original language	English (US)
Pages (from-to)	361-368
Number of pages	8
Journal	Journal of Immunological Methods
Volume	28
Issue number	3-4
DOIs	https://doi.org/10.1016/0022-1759(79)90201-1
State	Published - Jul 24 1979

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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title = "Binding of ovalbumin to mouse spleen cells with and without carbodiimide",

abstract = "We studied the binding of ovalbumin (OVA) to mouse spleen cells. In the presence or absence of carbodiimide (ECDI), uptake increased with greater cell numbers, increasing OVA concentration and increasing time up to 60 min. Between 60 and 210 μg OVA could be coupled with ECDI to 2 × 108 cells. In the absence of ECDI, however, OVA uptake was still appreciable, but appeared to be of low avidity as it did not occur in the presence of coupeting fetal calf serum. These experiments provide a basis for the use of protein coupled to autologous cells in the induction of immunologic tolerance.",

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T1 - Binding of ovalbumin to mouse spleen cells with and without carbodiimide

AU - Wetzig, Richard

AU - Hanson, Donald G.

AU - Miller, Stephen D.

AU - Claman, Henry N.

PY - 1979/7/24

Y1 - 1979/7/24

N2 - We studied the binding of ovalbumin (OVA) to mouse spleen cells. In the presence or absence of carbodiimide (ECDI), uptake increased with greater cell numbers, increasing OVA concentration and increasing time up to 60 min. Between 60 and 210 μg OVA could be coupled with ECDI to 2 × 108 cells. In the absence of ECDI, however, OVA uptake was still appreciable, but appeared to be of low avidity as it did not occur in the presence of coupeting fetal calf serum. These experiments provide a basis for the use of protein coupled to autologous cells in the induction of immunologic tolerance.

AB - We studied the binding of ovalbumin (OVA) to mouse spleen cells. In the presence or absence of carbodiimide (ECDI), uptake increased with greater cell numbers, increasing OVA concentration and increasing time up to 60 min. Between 60 and 210 μg OVA could be coupled with ECDI to 2 × 108 cells. In the absence of ECDI, however, OVA uptake was still appreciable, but appeared to be of low avidity as it did not occur in the presence of coupeting fetal calf serum. These experiments provide a basis for the use of protein coupled to autologous cells in the induction of immunologic tolerance.

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ER -

Binding of ovalbumin to mouse spleen cells with and without carbodiimide

Abstract

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