Bio-functionalized graphene-graphene oxide nanocomposite based electrochemical immunosensing

Priyanka Sharma, Satish K. Tuteja, Vijayender Bhalla, G. Shekhawat*, Vinayak P. Dravid, C. Raman Suri

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

75 Scopus citations

Abstract

We report a novel in-situ electrochemical synthesis approach for the formation of functionalized graphene-graphene oxide (fG-GO) nanocomposite on screen-printed electrodes (SPE). Electrochemically controlled nanocomposite film formation was studied by transmission electron microscopy (TEM) and Raman spectroscopy. Further insight into the nanocomposite has been accomplished by the Fourier transformed infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA) and X-ray diffraction (XRD) spectroscopy. Configured as a highly responsive screen-printed immunosensor, the fG-GO nanocomposite on SPE exhibits electrical and chemical synergies of the nano-hybrid functional construct by combining good electronic properties of functionalized graphene (fG) and the facile chemical functionality of graphene oxide (GO) for compatible bio-interface development using specific anti-diuron antibody. The enhanced electrical properties of nanocomposite biofilm demonstrated a significant increase in electrochemical signal response in a competitive inhibition immunoassay format for diuron detection, promising its potential applicability for ultra-sensitive detection of range of target analytes.

Original languageEnglish (US)
Pages (from-to)99-105
Number of pages7
JournalBiosensors and Bioelectronics
Volume39
Issue number1
DOIs
StatePublished - Jan 15 2013

Keywords

  • Diuron
  • Electrochemical immunosensor
  • Functionalised graphene-graphene oxide
  • Nanocomposites
  • Specific antibodies

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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