TY - JOUR
T1 - Bioactive glass 13-93 as a subchondral substrate for tissue-engineered osteochondral constructs
T2 - A pilot study
AU - Jayabalan, Prakash
AU - Tan, Andrea R.
AU - Rahaman, Mohammed N.
AU - Bal, B. Sonny
AU - Hung, Clark T.
AU - Cook, James L.
N1 - Funding Information:
One or more of the authors (PJ, BSB) have received funding for this research from a grant provided by the MU (University of Missouri) Research Council. Each author certifies that his or her institution has approved the animal protocol for this investigation and that all investigations were conducted in conformity with ethical principles of research. This work was performed at the Comparative Orthopaedic Laboratory, University of Missouri, and at the Cellular Engineering Laboratory, Columbia University.
PY - 2011/10
Y1 - 2011/10
N2 - Background: Replacement of diseased areas of the joint with tissue-engineered osteochondral grafts has shown potential in the treatment of osteoarthritis. Bioactive glasses are candidates for the osseous analog of these grafts. Questions/purposes: (1) Does Bioactive Glass 13-93 (BG 13-93) as a subchondral substrate improve collagen and glycosaminoglycan production in a tissue-engineered cartilage layer? (2) Does BG 13-93 as a culture medium supplement increase the collagen and glycosaminoglycan production and improve the mechanical properties in a tissue-engineered cartilage layer? Methods: In Study 1, bioactive glass samples (n = 4) were attached to a chondrocyte-seeded agarose layer to form an osteochondral construct, cultured for 6 weeks, and compared to controls. In Study 2, bioactive glass samples (n = 5) were cocultured with cell-seeded agarose for 6 weeks. The cell-seeded agarose layer was exposed to BG 13-93 either continuously or for the first or last 2 weeks in culture or had no exposure. Results: Osteochondral constructs with a BG 13-93 base had improved glycosaminoglycan deposition but less collagen II content. Agarose scaffolds that had a temporal exposure to BG 13-93 within the culture medium had improved mechanical and biochemical properties compared to continuous or no exposure. Conclusions: When used as a subchondral substrate, BG 13-93 did not improve biochemical properties compared to controls. However, as a culture medium supplement, BG 13-93 improved the biochemical and mechanical properties of a tissue-engineered cartilage layer. Clinical Relevance: BG 13-93 may not be suitable in osteochondral constructs but could have potential as a medium supplement for neocartilage formation.
AB - Background: Replacement of diseased areas of the joint with tissue-engineered osteochondral grafts has shown potential in the treatment of osteoarthritis. Bioactive glasses are candidates for the osseous analog of these grafts. Questions/purposes: (1) Does Bioactive Glass 13-93 (BG 13-93) as a subchondral substrate improve collagen and glycosaminoglycan production in a tissue-engineered cartilage layer? (2) Does BG 13-93 as a culture medium supplement increase the collagen and glycosaminoglycan production and improve the mechanical properties in a tissue-engineered cartilage layer? Methods: In Study 1, bioactive glass samples (n = 4) were attached to a chondrocyte-seeded agarose layer to form an osteochondral construct, cultured for 6 weeks, and compared to controls. In Study 2, bioactive glass samples (n = 5) were cocultured with cell-seeded agarose for 6 weeks. The cell-seeded agarose layer was exposed to BG 13-93 either continuously or for the first or last 2 weeks in culture or had no exposure. Results: Osteochondral constructs with a BG 13-93 base had improved glycosaminoglycan deposition but less collagen II content. Agarose scaffolds that had a temporal exposure to BG 13-93 within the culture medium had improved mechanical and biochemical properties compared to continuous or no exposure. Conclusions: When used as a subchondral substrate, BG 13-93 did not improve biochemical properties compared to controls. However, as a culture medium supplement, BG 13-93 improved the biochemical and mechanical properties of a tissue-engineered cartilage layer. Clinical Relevance: BG 13-93 may not be suitable in osteochondral constructs but could have potential as a medium supplement for neocartilage formation.
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U2 - 10.1007/s11999-011-1818-x
DO - 10.1007/s11999-011-1818-x
M3 - Article
C2 - 21365338
AN - SCOPUS:80054744781
VL - 469
SP - 2754
EP - 2763
JO - Clinical Orthopaedics and Related Research
JF - Clinical Orthopaedics and Related Research
SN - 0009-921X
IS - 10
ER -