Biochemical differentiation of nascent neurite junctions: unilateral localization of adheron components

Ho Ching Tiffany Tsui; William L. Klein

doi:10.1016/0165-3806(90)90277-6

Biochemical differentiation of nascent neurite junctions: unilateral localization of adheron components

Ho Ching Tiffany Tsui^*, William L. Klein

^*Corresponding author for this work

Neurobiology

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Previous work showed that adhesive contacts made by growth cone filopodia involve extracellular filaments that can be labeled with an antiserum to adherons (adhesion-promoting complexes in conditioned medium). Here, adheron antigens were found to be differentially expressed by particular cell types and synaptic layers during chick retina development, and this differential expression at the cellular level was retained in culture. When applied to living cells, adheron antibodies characteristically patched at filopodial junctions. Monospecific antibodies to purpurin and a heparan sulphate proteoglycan (HSPG), two components of adherons, labeled a subpopulation of junctions. Most interestingly, anti-purpurin and anti-HSPG bound only to one end of adhesive filaments. Such localization to discrete microdomains provides support for a heterotypic adhesive mechanism in junction formation.

Original language	English (US)
Pages (from-to)	205-216
Number of pages	12
Journal	Developmental Brain Research
Volume	51
Issue number	2
DOIs	https://doi.org/10.1016/0165-3806(90)90277-6
State	Published - Feb 1 1990

Funding

Acknowledgement. This work was supported by NIH Grant NS21088 to W.L.K.

Keywords

Adhesion
Filopodia
Growth cone
Heparan sulphate proteoglycan
Purpurin
Retina
Synapse

ASJC Scopus subject areas

Developmental Neuroscience
Developmental Biology

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10.1016/0165-3806(90)90277-6

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title = "Biochemical differentiation of nascent neurite junctions: unilateral localization of adheron components",

abstract = "Previous work showed that adhesive contacts made by growth cone filopodia involve extracellular filaments that can be labeled with an antiserum to adherons (adhesion-promoting complexes in conditioned medium). Here, adheron antigens were found to be differentially expressed by particular cell types and synaptic layers during chick retina development, and this differential expression at the cellular level was retained in culture. When applied to living cells, adheron antibodies characteristically patched at filopodial junctions. Monospecific antibodies to purpurin and a heparan sulphate proteoglycan (HSPG), two components of adherons, labeled a subpopulation of junctions. Most interestingly, anti-purpurin and anti-HSPG bound only to one end of adhesive filaments. Such localization to discrete microdomains provides support for a heterotypic adhesive mechanism in junction formation.",

keywords = "Adhesion, Filopodia, Growth cone, Heparan sulphate proteoglycan, Purpurin, Retina, Synapse",

author = "Tsui, {Ho Ching Tiffany} and Klein, {William L.}",

note = "Funding Information: Acknowledgement. This work was supported by NIH Grant NS21088 to W.L.K.",

year = "1990",

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doi = "10.1016/0165-3806(90)90277-6",

language = "English (US)",

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TY - JOUR

T1 - Biochemical differentiation of nascent neurite junctions

T2 - unilateral localization of adheron components

AU - Tsui, Ho Ching Tiffany

AU - Klein, William L.

N1 - Funding Information: Acknowledgement. This work was supported by NIH Grant NS21088 to W.L.K.

PY - 1990/2/1

Y1 - 1990/2/1

N2 - Previous work showed that adhesive contacts made by growth cone filopodia involve extracellular filaments that can be labeled with an antiserum to adherons (adhesion-promoting complexes in conditioned medium). Here, adheron antigens were found to be differentially expressed by particular cell types and synaptic layers during chick retina development, and this differential expression at the cellular level was retained in culture. When applied to living cells, adheron antibodies characteristically patched at filopodial junctions. Monospecific antibodies to purpurin and a heparan sulphate proteoglycan (HSPG), two components of adherons, labeled a subpopulation of junctions. Most interestingly, anti-purpurin and anti-HSPG bound only to one end of adhesive filaments. Such localization to discrete microdomains provides support for a heterotypic adhesive mechanism in junction formation.

AB - Previous work showed that adhesive contacts made by growth cone filopodia involve extracellular filaments that can be labeled with an antiserum to adherons (adhesion-promoting complexes in conditioned medium). Here, adheron antigens were found to be differentially expressed by particular cell types and synaptic layers during chick retina development, and this differential expression at the cellular level was retained in culture. When applied to living cells, adheron antibodies characteristically patched at filopodial junctions. Monospecific antibodies to purpurin and a heparan sulphate proteoglycan (HSPG), two components of adherons, labeled a subpopulation of junctions. Most interestingly, anti-purpurin and anti-HSPG bound only to one end of adhesive filaments. Such localization to discrete microdomains provides support for a heterotypic adhesive mechanism in junction formation.

KW - Adhesion

KW - Filopodia

KW - Growth cone

KW - Heparan sulphate proteoglycan

KW - Purpurin

KW - Retina

KW - Synapse

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DO - 10.1016/0165-3806(90)90277-6

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SN - 0165-3806

VL - 51

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JO - Developmental Brain Research

JF - Developmental Brain Research

IS - 2

ER -

Biochemical differentiation of nascent neurite junctions: unilateral localization of adheron components

Abstract

Funding

Keywords

ASJC Scopus subject areas

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