Biocompatibility of methylcellulose-based constructs designed for intracerebral gelation following experimental traumatic brain injury

Matthew C. Tate, Deborah A. Shear, Stuart W. Hoffman, Donald G. Stein, Michelle C. LaPlaca*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

195 Scopus citations

Abstract

Tissue engineering in the post-injury brain represents a promising option for cellular replacement and rescue, providing a cell scaffold for either transplanted or resident cells. We have characterized the use of methylcellulose (MC) as a scaffolding material, whose concentration and solvent were varied to manipulate its physical properties. MC solutions were produced to exhibit low viscosity at 23°C and form a soft gel at 37°C, thereby making MC attractive for minimally invasive procedures in vivo. Degradation and swelling studies in vitro demonstrated a small amount of initial polymer erosion followed by relative polymer stability over the 2-week period tested as well as increased hydrogel mass due to solvent uptake. Concentrations up to 8% did not elicit cell death in primary rat astrocytes or neurons at 1 or 7 days. Acellular 2% MC (30μl) was microinjected into the brains of rats 1 week after cortical impact injury (velocity=3m/s, depth=2mm) and examined at 2 days (n=8; n=3, vehicle injected) and 2 weeks (n=5; n=3, vehicle injected). The presence of MC did not alter the size of the injury cavity or change the patterns of gliosis as compared to injured, vehicle-injected rats (detected using antibodies against GFAP and ED1). Collectively, these data indicate that MC is well suited as a biocompatible injectable scaffold for the repair of defects in the brain.

Original languageEnglish (US)
Pages (from-to)1113-1123
Number of pages11
JournalBiomaterials
Volume22
Issue number10
DOIs
StatePublished - 2001

Keywords

  • Cell scaffold
  • Methylcellulose
  • Neural tissue engineering
  • Neurotrauma
  • Traumatic brain injury

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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