Bioinformatic comparisons and tissue expression of the neuronal nitric oxide synthase (nNOS) gene from the red drum (Sciaenops ocellatus)

Libin Zhou; Ru Bai; Jianxiao Tian; Xiaochun Liu; Danqi Lu; Pei Zhu; Yun Liu; Lujiao Zeng; Wenna Luo; Yong Zhang; Anli Wang

doi:10.1016/j.fsi.2009.07.010

Bioinformatic comparisons and tissue expression of the neuronal nitric oxide synthase (nNOS) gene from the red drum (Sciaenops ocellatus)

Libin Zhou, Ru Bai, Jianxiao Tian, Xiaochun Liu, Danqi Lu, Pei Zhu, Yun Liu, Lujiao Zeng, Wenna Luo, Yong Zhang, Anli Wang^*

^*Corresponding author for this work

Biochemistry and Molecular Genetics

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

The full length cDNA sequence for neuronal nitric oxide synthase (nNOS) gene from red drum (Sciaenops ocellatus) has been cloned, subjected to bioinformatic analysis, and examined for expression in different tissues. Red drum nNOS showed high identity to nNOS of mammals and other fish species. Notably, a unique 7-aa insertion was found in the important catalytic sites of the NO synthase domain, possibly affecting the function of red drum nNOS. Furthermore, this nNOS was expressed not only in brain but also in most of the internal organs including liver, intestine, spleen, head kidney and thymus.

Original language	English (US)
Pages (from-to)	577-584
Number of pages	8
Journal	Fish and Shellfish Immunology
Volume	27
Issue number	4
DOIs	https://doi.org/10.1016/j.fsi.2009.07.010
State	Published - Oct 2009

Keywords

Expression pattern
Neuronal NO synthase (nNOS)

ASJC Scopus subject areas

Environmental Chemistry
Aquatic Science

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10.1016/j.fsi.2009.07.010

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title = "Bioinformatic comparisons and tissue expression of the neuronal nitric oxide synthase (nNOS) gene from the red drum (Sciaenops ocellatus)",

abstract = "The full length cDNA sequence for neuronal nitric oxide synthase (nNOS) gene from red drum (Sciaenops ocellatus) has been cloned, subjected to bioinformatic analysis, and examined for expression in different tissues. Red drum nNOS showed high identity to nNOS of mammals and other fish species. Notably, a unique 7-aa insertion was found in the important catalytic sites of the NO synthase domain, possibly affecting the function of red drum nNOS. Furthermore, this nNOS was expressed not only in brain but also in most of the internal organs including liver, intestine, spleen, head kidney and thymus.",

keywords = "Expression pattern, Neuronal NO synthase (nNOS)",

author = "Libin Zhou and Ru Bai and Jianxiao Tian and Xiaochun Liu and Danqi Lu and Pei Zhu and Yun Liu and Lujiao Zeng and Wenna Luo and Yong Zhang and Anli Wang",

note = "Funding Information: This work was supported by Foundation of Science and Technology Thriving Ocean and Fishery (A200501 G01), National Key Technology R&D Program (2007BAD29B04), Foundation of Science and Technology of Guangdong Province (2007B020708013).",

year = "2009",

month = oct,

doi = "10.1016/j.fsi.2009.07.010",

language = "English (US)",

volume = "27",

pages = "577--584",

journal = "Fish and Shellfish Immunology",

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T1 - Bioinformatic comparisons and tissue expression of the neuronal nitric oxide synthase (nNOS) gene from the red drum (Sciaenops ocellatus)

AU - Zhou, Libin

AU - Bai, Ru

AU - Tian, Jianxiao

AU - Liu, Xiaochun

AU - Lu, Danqi

AU - Zhu, Pei

AU - Liu, Yun

AU - Zeng, Lujiao

AU - Luo, Wenna

AU - Zhang, Yong

AU - Wang, Anli

N1 - Funding Information: This work was supported by Foundation of Science and Technology Thriving Ocean and Fishery (A200501 G01), National Key Technology R&D Program (2007BAD29B04), Foundation of Science and Technology of Guangdong Province (2007B020708013).

PY - 2009/10

Y1 - 2009/10

N2 - The full length cDNA sequence for neuronal nitric oxide synthase (nNOS) gene from red drum (Sciaenops ocellatus) has been cloned, subjected to bioinformatic analysis, and examined for expression in different tissues. Red drum nNOS showed high identity to nNOS of mammals and other fish species. Notably, a unique 7-aa insertion was found in the important catalytic sites of the NO synthase domain, possibly affecting the function of red drum nNOS. Furthermore, this nNOS was expressed not only in brain but also in most of the internal organs including liver, intestine, spleen, head kidney and thymus.

AB - The full length cDNA sequence for neuronal nitric oxide synthase (nNOS) gene from red drum (Sciaenops ocellatus) has been cloned, subjected to bioinformatic analysis, and examined for expression in different tissues. Red drum nNOS showed high identity to nNOS of mammals and other fish species. Notably, a unique 7-aa insertion was found in the important catalytic sites of the NO synthase domain, possibly affecting the function of red drum nNOS. Furthermore, this nNOS was expressed not only in brain but also in most of the internal organs including liver, intestine, spleen, head kidney and thymus.

KW - Expression pattern

KW - Neuronal NO synthase (nNOS)

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JO - Fish and Shellfish Immunology

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ER -

Bioinformatic comparisons and tissue expression of the neuronal nitric oxide synthase (nNOS) gene from the red drum (Sciaenops ocellatus)

Abstract

Keywords

ASJC Scopus subject areas

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