@inbook{4cc9fab4c26f46fa9478e529f2d2b900,
title = "Bioinformatics analysis of top-down mass spectrometry data with proSight lite",
abstract = "Traditional bottom-up mass spectrometry-based proteomics relies on the use of an enzyme, often trypsin, to generate small peptides (typically < 25 amino acids long). In top-down proteomics, proteins remain intact and are directly measured within the mass spectrometer. This technique, while inherently simpler than bottom-up proteomics, generates data which must be processed and analyzed using software tools “purpose-built” for the job. In this chapter, we will show the analysis of intact protein spectra through deconvolution, deisotoping, and searching with ProSight Lite, a free, vendor-agnostic tool for the analysis of top-down mass spectrometry data. We will illustrate with two examples of intact protein fragmentation spectra and discuss the iterative use of the software to characterize proteoforms and discover the sites of post-translational modifications.",
keywords = "Bioinformatics, Intact protein, Mass spectrometry, ProSight lite, Proteomics, Top-down",
author = "Dehart, {Caroline J.} and Fellers, {Ryan T.} and Luca Fornelli and Kelleher, {Neil L.} and Thomas, {Paul M.}",
note = "Funding Information: This work was supported by Award No. P41GM108569 from the National Institute of General Medical Sciences The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Publisher Copyright: {\textcopyright} Springer Science+Business Media LLC 2017.",
year = "2017",
doi = "10.1007/978-1-4939-6783-4_18",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "381--394",
booktitle = "Methods in Molecular Biology",
}