Biologically active protein nanoarrays generated using parallel dip-pen nanolithography

The generation of biologically active protein nanoarrays using parallel dip-pen nanolithography (DPN), was investigated. To generate nanoscale arrays of amine-reactive dot features on a gold substrate, which was prepared using a 26-pen array was coated with NHSC₁₁SH by immersing it in a 10mM NHSC₁₁SH acetonitrile solution for 10 s. The DPN-generated NHSC ₁₁SH dot arrays served as a template for the immobilization of protein A/G. Through the use of the affinity binding of the antibodies on protein A/G, biologically active antibody nanoarrays can e generated over macroscopic distances through parallel DPN, demonstrating the versatility of this approach for making similar antibody structures in a relatively high-throughput manner. The results show that researchers in nanoscience and biology will consider new ways of using such patterned interfaces to probe biological systems.