Bioluminescent nanosensors for protease detection based upon gold nanoparticle-luciferase conjugates

Young Pil Kim, Weston L. Daniel, Zuyong Xia, Hexin Xie, Chad A. Mirkin, Jianghong Rao*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

86 Scopus citations

Abstract

This communication reports the use of click chemistry to site-specifically conjugate bioluminescent Renilla luciferase proteins to gold nanoparticles (Au NPs) for sensing protease activity. The bioluminescent emission from luciferase was efficiently quenched by Au NPs, but significantly recovered after the proteolytic cleavage.

Original languageEnglish (US)
Pages (from-to)76-78
Number of pages3
JournalChemical Communications
Volume46
Issue number1
DOIs
StatePublished - 2010

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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