Bioluminescent nanosensors for protease detection based upon gold nanoparticle-luciferase conjugates

Young Pil Kim; Weston L. Daniel; Zuyong Xia; Hexin Xie; Chad A. Mirkin; Jianghong Rao

doi:10.1039/b915612g

Bioluminescent nanosensors for protease detection based upon gold nanoparticle-luciferase conjugates

Young Pil Kim, Weston L. Daniel, Zuyong Xia, Hexin Xie, Chad A. Mirkin, Jianghong Rao^*

^*Corresponding author for this work

Chemistry

Research output: Contribution to journal › Article › peer-review

86 Scopus citations

Abstract

This communication reports the use of click chemistry to site-specifically conjugate bioluminescent Renilla luciferase proteins to gold nanoparticles (Au NPs) for sensing protease activity. The bioluminescent emission from luciferase was efficiently quenched by Au NPs, but significantly recovered after the proteolytic cleavage.

Original language	English (US)
Pages (from-to)	76-78
Number of pages	3
Journal	Chemical Communications
Volume	46
Issue number	1
DOIs	https://doi.org/10.1039/b915612g
State	Published - 2010

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

Access to Document

10.1039/b915612g

Fingerprint Dive into the research topics of 'Bioluminescent nanosensors for protease detection based upon gold nanoparticle-luciferase conjugates'. Together they form a unique fingerprint.

Cite this

@article{8c3d03233576412db28cdb54759d14e5,

title = "Bioluminescent nanosensors for protease detection based upon gold nanoparticle-luciferase conjugates",

abstract = "This communication reports the use of click chemistry to site-specifically conjugate bioluminescent Renilla luciferase proteins to gold nanoparticles (Au NPs) for sensing protease activity. The bioluminescent emission from luciferase was efficiently quenched by Au NPs, but significantly recovered after the proteolytic cleavage.",

author = "Kim, {Young Pil} and Daniel, {Weston L.} and Zuyong Xia and Hexin Xie and Mirkin, {Chad A.} and Jianghong Rao",

year = "2010",

doi = "10.1039/b915612g",

language = "English (US)",

volume = "46",

pages = "76--78",

journal = "Chemical Communications",

issn = "1359-7345",

number = "1",

}

TY - JOUR

T1 - Bioluminescent nanosensors for protease detection based upon gold nanoparticle-luciferase conjugates

AU - Kim, Young Pil

AU - Daniel, Weston L.

AU - Xia, Zuyong

AU - Xie, Hexin

AU - Mirkin, Chad A.

AU - Rao, Jianghong

PY - 2010

Y1 - 2010

N2 - This communication reports the use of click chemistry to site-specifically conjugate bioluminescent Renilla luciferase proteins to gold nanoparticles (Au NPs) for sensing protease activity. The bioluminescent emission from luciferase was efficiently quenched by Au NPs, but significantly recovered after the proteolytic cleavage.

AB - This communication reports the use of click chemistry to site-specifically conjugate bioluminescent Renilla luciferase proteins to gold nanoparticles (Au NPs) for sensing protease activity. The bioluminescent emission from luciferase was efficiently quenched by Au NPs, but significantly recovered after the proteolytic cleavage.

UR - http://www.scopus.com/inward/record.url?scp=72149096051&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=72149096051&partnerID=8YFLogxK

U2 - 10.1039/b915612g

DO - 10.1039/b915612g

M3 - Article

C2 - 20024298

AN - SCOPUS:72149096051

VL - 46

SP - 76

EP - 78

JO - Chemical Communications

JF - Chemical Communications

SN - 1359-7345

IS - 1

ER -