Biomarkers of eosinophil involvement in allergic and eosinophilic diseases: Review of phenotypic and serum markers including a novel assay to quantify levels of soluble Siglec-8

Ho Jeong Na, Robert G. Hamilton, Amy D. Klion, Bruce S. Bochner*

*Corresponding author for this work

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

There remains considerable controversy in the management of eosinophilic disorders, mainly due to a paucity of information regarding the clinical interpretation of total blood eosinophil counts versus surface activation markers versus eosinophil-derived or eosinophil-influencing mediator levels. Regrettably, few tests have been validated that define a unique clinical or prognostic phenotype that is more useful than simply monitoring total blood eosinophil counts. In this manuscript, phenotypic (cell surface) markers, along with serum and tissue-based markers that have been examined in the context of disease activity, are reviewed. We also report the development of a novel assay for detecting soluble Siglec-8 (sSiglec-8), a protein likely derived largely from eosinophils, as a potential serum biomarker. The assay consists of a competitive ELISA using a recombinant Siglec-8-Fc fusion protein. The goal of this preliminary study was to determine if sSiglec-8 is a useful biomarker that differentiates among patients with various eosinophil-associated diseases. In the final analysis, it is fair to say that further research is sorely needed to fully understand and validate the utility of various biomarkers, including sSiglec-8, before their use in clinical practice can be recommended with confidence.

Original languageEnglish (US)
Pages (from-to)39-46
Number of pages8
JournalJournal of Immunological Methods
Volume383
Issue number1-2
DOIs
StatePublished - Sep 28 2012

Keywords

  • ELISA
  • Eosinophil
  • Granule proteins
  • Soluble Siglec-8

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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