Biomimetic high density lipoprotein nanoparticles for nucleic acid delivery

Kaylin M. McMahon, R. Kannan Mutharasan, Sushant Tripathy, Dorina Veliceasa, Mariana Bobeica, Dale K. Shumaker, Andrea J. Luthi, Brian T. Helfand, Hossein Ardehali, Chad A. Mirkin, Olga Volpert, C. Shad Thaxton

Research output: Contribution to journalArticlepeer-review

96 Scopus citations

Abstract

We report a gold nanoparticle-templated high density lipoprotein (HDL AuNP) platform for gene therapy that combines lipid-based nucleic acid transfection strategies with HDL biomimicry. For proof-of-concept, HDL AuNPs are shown to adsorb antisense cholesterylated DNA. The conjugates are internalized by human cells, can be tracked within cells using transmission electron microscopy, and regulate target gene expression. Overall, the ability to directly image the AuNP core within cells, the chemical tailorability of the HDL AuNP platform, and the potential for cell-specific targeting afforded by HDL biomimicry make this platform appealing for nucleic acid delivery.

Original languageEnglish (US)
Pages (from-to)1208-1214
Number of pages7
JournalNano letters
Volume11
Issue number3
DOIs
StatePublished - Mar 9 2011

Keywords

  • High density lipoprotein
  • biomimetic
  • gene therapy
  • gold nanoparticle
  • nucleic acid delivery

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

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