Biomimetic high density lipoprotein nanoparticles for nucleic acid delivery

Kaylin M. McMahon, R. Kannan Mutharasan, Sushant Tripathy, Dorina Veliceasa, Mariana Bobeica, Dale K. Shumaker, Andrea J. Luthi, Brian T. Helfand, Hossein Ardehali, Chad A. Mirkin, Olga Volpert, C. Shad Thaxton

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

We report a gold nanoparticle-templated high density lipoprotein (HDL AuNP) platform for gene therapy that combines lipid-based nucleic acid transfection strategies with HDL biomimicry. For proof-of-concept, HDL AuNPs are shown to adsorb antisense cholesterylated DNA. The conjugates are internalized by human cells, can be tracked within cells using transmission electron microscopy, and regulate target gene expression. Overall, the ability to directly image the AuNP core within cells, the chemical tailorability of the HDL AuNP platform, and the potential for cell-specific targeting afforded by HDL biomimicry make this platform appealing for nucleic acid delivery.

Original languageEnglish (US)
Pages (from-to)1208-1214
Number of pages7
JournalNano Letters
Volume11
Issue number3
DOIs
StatePublished - Mar 9 2011

Fingerprint

lipoproteins
hardware description languages
Lipoproteins
Nucleic acids
biomimetics
Biomimetics
nucleic acids
HDL Lipoproteins
Nucleic Acids
delivery
Antisense DNA
Nanoparticles
Gene therapy
nanoparticles
Gene expression
Gold
platforms
Lipids
DNA
Cells

Keywords

  • High density lipoprotein
  • biomimetic
  • gene therapy
  • gold nanoparticle
  • nucleic acid delivery

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

Cite this

McMahon, Kaylin M. ; Mutharasan, R. Kannan ; Tripathy, Sushant ; Veliceasa, Dorina ; Bobeica, Mariana ; Shumaker, Dale K. ; Luthi, Andrea J. ; Helfand, Brian T. ; Ardehali, Hossein ; Mirkin, Chad A. ; Volpert, Olga ; Thaxton, C. Shad. / Biomimetic high density lipoprotein nanoparticles for nucleic acid delivery. In: Nano Letters. 2011 ; Vol. 11, No. 3. pp. 1208-1214.
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McMahon, KM, Mutharasan, RK, Tripathy, S, Veliceasa, D, Bobeica, M, Shumaker, DK, Luthi, AJ, Helfand, BT, Ardehali, H, Mirkin, CA, Volpert, O & Thaxton, CS 2011, 'Biomimetic high density lipoprotein nanoparticles for nucleic acid delivery', Nano Letters, vol. 11, no. 3, pp. 1208-1214. https://doi.org/10.1021/nl1041947

Biomimetic high density lipoprotein nanoparticles for nucleic acid delivery. / McMahon, Kaylin M.; Mutharasan, R. Kannan; Tripathy, Sushant; Veliceasa, Dorina; Bobeica, Mariana; Shumaker, Dale K.; Luthi, Andrea J.; Helfand, Brian T.; Ardehali, Hossein; Mirkin, Chad A.; Volpert, Olga; Thaxton, C. Shad.

In: Nano Letters, Vol. 11, No. 3, 09.03.2011, p. 1208-1214.

Research output: Contribution to journalArticle

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AU - McMahon, Kaylin M.

AU - Mutharasan, R. Kannan

AU - Tripathy, Sushant

AU - Veliceasa, Dorina

AU - Bobeica, Mariana

AU - Shumaker, Dale K.

AU - Luthi, Andrea J.

AU - Helfand, Brian T.

AU - Ardehali, Hossein

AU - Mirkin, Chad A.

AU - Volpert, Olga

AU - Thaxton, C. Shad

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McMahon KM, Mutharasan RK, Tripathy S, Veliceasa D, Bobeica M, Shumaker DK et al. Biomimetic high density lipoprotein nanoparticles for nucleic acid delivery. Nano Letters. 2011 Mar 9;11(3):1208-1214. https://doi.org/10.1021/nl1041947