Biomimetic high density lipoprotein nanoparticles for nucleic acid delivery

Kaylin M. McMahon; R. Kannan Mutharasan; Sushant Tripathy; Dorina Veliceasa; Mariana Bobeica; Dale K. Shumaker; Andrea J. Luthi; Brian T. Helfand; Hossein Ardehali; Chad A. Mirkin; Olga Volpert; C. Shad Thaxton

doi:10.1021/nl1041947

Biomimetic high density lipoprotein nanoparticles for nucleic acid delivery

Kaylin M. McMahon, R. Kannan Mutharasan, Sushant Tripathy, Dorina Veliceasa, Mariana Bobeica, Dale K. Shumaker, Andrea J. Luthi, Brian T. Helfand, Hossein Ardehali, Chad A. Mirkin, Olga Volpert, C. Shad Thaxton

Research output: Contribution to journal › Article › peer-review

114 Scopus citations

Abstract

We report a gold nanoparticle-templated high density lipoprotein (HDL AuNP) platform for gene therapy that combines lipid-based nucleic acid transfection strategies with HDL biomimicry. For proof-of-concept, HDL AuNPs are shown to adsorb antisense cholesterylated DNA. The conjugates are internalized by human cells, can be tracked within cells using transmission electron microscopy, and regulate target gene expression. Overall, the ability to directly image the AuNP core within cells, the chemical tailorability of the HDL AuNP platform, and the potential for cell-specific targeting afforded by HDL biomimicry make this platform appealing for nucleic acid delivery.

Original language	English (US)
Pages (from-to)	1208-1214
Number of pages	7
Journal	Nano letters
Volume	11
Issue number	3
DOIs	https://doi.org/10.1021/nl1041947
State	Published - Mar 9 2011

Keywords

High density lipoprotein
biomimetic
gene therapy
gold nanoparticle
nucleic acid delivery

ASJC Scopus subject areas

Condensed Matter Physics
Mechanical Engineering
Bioengineering
Chemistry(all)
Materials Science(all)

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10.1021/nl1041947

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title = "Biomimetic high density lipoprotein nanoparticles for nucleic acid delivery",

abstract = "We report a gold nanoparticle-templated high density lipoprotein (HDL AuNP) platform for gene therapy that combines lipid-based nucleic acid transfection strategies with HDL biomimicry. For proof-of-concept, HDL AuNPs are shown to adsorb antisense cholesterylated DNA. The conjugates are internalized by human cells, can be tracked within cells using transmission electron microscopy, and regulate target gene expression. Overall, the ability to directly image the AuNP core within cells, the chemical tailorability of the HDL AuNP platform, and the potential for cell-specific targeting afforded by HDL biomimicry make this platform appealing for nucleic acid delivery.",

keywords = "High density lipoprotein, biomimetic, gene therapy, gold nanoparticle, nucleic acid delivery",

author = "McMahon, {Kaylin M.} and Mutharasan, {R. Kannan} and Sushant Tripathy and Dorina Veliceasa and Mariana Bobeica and Shumaker, {Dale K.} and Luthi, {Andrea J.} and Helfand, {Brian T.} and Hossein Ardehali and Mirkin, {Chad A.} and Olga Volpert and Thaxton, {C. Shad}",

year = "2011",

month = mar,

day = "9",

doi = "10.1021/nl1041947",

language = "English (US)",

volume = "11",

pages = "1208--1214",

journal = "Nano Letters",

issn = "1530-6984",

publisher = "American Chemical Society",

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TY - JOUR

T1 - Biomimetic high density lipoprotein nanoparticles for nucleic acid delivery

AU - McMahon, Kaylin M.

AU - Mutharasan, R. Kannan

AU - Tripathy, Sushant

AU - Veliceasa, Dorina

AU - Bobeica, Mariana

AU - Shumaker, Dale K.

AU - Luthi, Andrea J.

AU - Helfand, Brian T.

AU - Ardehali, Hossein

AU - Mirkin, Chad A.

AU - Volpert, Olga

AU - Thaxton, C. Shad

PY - 2011/3/9

Y1 - 2011/3/9

N2 - We report a gold nanoparticle-templated high density lipoprotein (HDL AuNP) platform for gene therapy that combines lipid-based nucleic acid transfection strategies with HDL biomimicry. For proof-of-concept, HDL AuNPs are shown to adsorb antisense cholesterylated DNA. The conjugates are internalized by human cells, can be tracked within cells using transmission electron microscopy, and regulate target gene expression. Overall, the ability to directly image the AuNP core within cells, the chemical tailorability of the HDL AuNP platform, and the potential for cell-specific targeting afforded by HDL biomimicry make this platform appealing for nucleic acid delivery.

AB - We report a gold nanoparticle-templated high density lipoprotein (HDL AuNP) platform for gene therapy that combines lipid-based nucleic acid transfection strategies with HDL biomimicry. For proof-of-concept, HDL AuNPs are shown to adsorb antisense cholesterylated DNA. The conjugates are internalized by human cells, can be tracked within cells using transmission electron microscopy, and regulate target gene expression. Overall, the ability to directly image the AuNP core within cells, the chemical tailorability of the HDL AuNP platform, and the potential for cell-specific targeting afforded by HDL biomimicry make this platform appealing for nucleic acid delivery.

KW - High density lipoprotein

KW - biomimetic

KW - gene therapy

KW - gold nanoparticle

KW - nucleic acid delivery

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JF - Nano Letters

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Biomimetic high density lipoprotein nanoparticles for nucleic acid delivery

Abstract

Keywords

ASJC Scopus subject areas

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