Biopsy diagnosis of Creutzfeldt-Jakob disease by western blot: A case report

R. J. Castellani, P. Parchi, L. Madoff, P. Gambetti, P. Mckeever

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Creutzfeldt-Jakob disease is a clinically and pathologically heterogeneous disorder that often requires brain biopsy for definitive diagnosis. We report the case of a 62-year-old man who underwent brain biopsy for progressive neurological deterioration. Histopathologically, there was minimal spongiform change that could not be unequivocally attributed to Creutzfeldt-Jakob disease. A 16 mg portion of gray matter saved frozen was subsequently analyzed by Western blot and showed definitive protease- resistant prion protein. This case illustrates applicability, ease in interpretation, and accuracy of Western blot analysis for protease-resistant prion protein in small brain biopsy specimens. Given the importance of accurate diagnosis in suspected prion disease, we recommend that a small portion of tissue from any brain biopsy performed in this setting be kept frozen for possible biochemical studies.

Original languageEnglish (US)
Pages (from-to)623-626
Number of pages4
JournalHuman pathology
Issue number5
StatePublished - 1997


  • Creutzfeldt-Jakob disease
  • electron microscopy
  • immunohistochemistry
  • prion protein
  • Western blot

ASJC Scopus subject areas

  • Pathology and Forensic Medicine


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