Bioreactor design for perfusion-based, highly vascularized organ regeneration

Brent M. Bijonowski, William M Miller, Jason A Wertheim

Research output: Contribution to journalReview article

20 Citations (Scopus)

Abstract

The production of bioartificial or laboratory-grown organs is a growing field centered on developing replacement organs and tissues to restore body function and providing a potential solution to the shortage of donor organs for transplantation. With the entry of engineered planar tissues, such as bladder and trachea, into clinical studies, an increasing focus is being given to designing complex, three-dimensional solid organs. As tissues become larger, thicker and more complex, the vascular network becomes crucial for supplying nutrients and maintaining viability and growth of the neo-organ. Perfusion decellularization, the process of removing cells from an entire organ, leaves the matrix of the vascular network intact. Organ engineering requires a delicate process of decellularization, sterilization, reseeding with appropriate cells, and organ maturation and stimulation to ensure optimal development. The design of bioreactors to facilitate this sequence of events has been refined to the extent that some bioartificial organs grown in these systems have been transplanted into recipient animals with sustained, though limited, function. This review focuses on the state-of-art in bioreactor development for perfusion-based bioartificial organs and highlights specific design components in need of further refinement.

Original languageEnglish (US)
Pages (from-to)32-40
Number of pages9
JournalCurrent Opinion in Chemical Engineering
Volume2
Issue number1
DOIs
StatePublished - Jan 1 2013

Fingerprint

Bioreactors
Tissue
Nutrients
Animals

ASJC Scopus subject areas

  • Energy(all)

Cite this

@article{946a58a4946045708fbe2314efce4ce7,
title = "Bioreactor design for perfusion-based, highly vascularized organ regeneration",
abstract = "The production of bioartificial or laboratory-grown organs is a growing field centered on developing replacement organs and tissues to restore body function and providing a potential solution to the shortage of donor organs for transplantation. With the entry of engineered planar tissues, such as bladder and trachea, into clinical studies, an increasing focus is being given to designing complex, three-dimensional solid organs. As tissues become larger, thicker and more complex, the vascular network becomes crucial for supplying nutrients and maintaining viability and growth of the neo-organ. Perfusion decellularization, the process of removing cells from an entire organ, leaves the matrix of the vascular network intact. Organ engineering requires a delicate process of decellularization, sterilization, reseeding with appropriate cells, and organ maturation and stimulation to ensure optimal development. The design of bioreactors to facilitate this sequence of events has been refined to the extent that some bioartificial organs grown in these systems have been transplanted into recipient animals with sustained, though limited, function. This review focuses on the state-of-art in bioreactor development for perfusion-based bioartificial organs and highlights specific design components in need of further refinement.",
author = "Bijonowski, {Brent M.} and Miller, {William M} and Wertheim, {Jason A}",
year = "2013",
month = "1",
day = "1",
doi = "10.1016/j.coche.2012.12.001",
language = "English (US)",
volume = "2",
pages = "32--40",
journal = "Current Opinion in Chemical Engineering",
issn = "2211-3398",
publisher = "Elsevier BV",
number = "1",

}

Bioreactor design for perfusion-based, highly vascularized organ regeneration. / Bijonowski, Brent M.; Miller, William M; Wertheim, Jason A.

In: Current Opinion in Chemical Engineering, Vol. 2, No. 1, 01.01.2013, p. 32-40.

Research output: Contribution to journalReview article

TY - JOUR

T1 - Bioreactor design for perfusion-based, highly vascularized organ regeneration

AU - Bijonowski, Brent M.

AU - Miller, William M

AU - Wertheim, Jason A

PY - 2013/1/1

Y1 - 2013/1/1

N2 - The production of bioartificial or laboratory-grown organs is a growing field centered on developing replacement organs and tissues to restore body function and providing a potential solution to the shortage of donor organs for transplantation. With the entry of engineered planar tissues, such as bladder and trachea, into clinical studies, an increasing focus is being given to designing complex, three-dimensional solid organs. As tissues become larger, thicker and more complex, the vascular network becomes crucial for supplying nutrients and maintaining viability and growth of the neo-organ. Perfusion decellularization, the process of removing cells from an entire organ, leaves the matrix of the vascular network intact. Organ engineering requires a delicate process of decellularization, sterilization, reseeding with appropriate cells, and organ maturation and stimulation to ensure optimal development. The design of bioreactors to facilitate this sequence of events has been refined to the extent that some bioartificial organs grown in these systems have been transplanted into recipient animals with sustained, though limited, function. This review focuses on the state-of-art in bioreactor development for perfusion-based bioartificial organs and highlights specific design components in need of further refinement.

AB - The production of bioartificial or laboratory-grown organs is a growing field centered on developing replacement organs and tissues to restore body function and providing a potential solution to the shortage of donor organs for transplantation. With the entry of engineered planar tissues, such as bladder and trachea, into clinical studies, an increasing focus is being given to designing complex, three-dimensional solid organs. As tissues become larger, thicker and more complex, the vascular network becomes crucial for supplying nutrients and maintaining viability and growth of the neo-organ. Perfusion decellularization, the process of removing cells from an entire organ, leaves the matrix of the vascular network intact. Organ engineering requires a delicate process of decellularization, sterilization, reseeding with appropriate cells, and organ maturation and stimulation to ensure optimal development. The design of bioreactors to facilitate this sequence of events has been refined to the extent that some bioartificial organs grown in these systems have been transplanted into recipient animals with sustained, though limited, function. This review focuses on the state-of-art in bioreactor development for perfusion-based bioartificial organs and highlights specific design components in need of further refinement.

UR - http://www.scopus.com/inward/record.url?scp=84875265313&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84875265313&partnerID=8YFLogxK

U2 - 10.1016/j.coche.2012.12.001

DO - 10.1016/j.coche.2012.12.001

M3 - Review article

VL - 2

SP - 32

EP - 40

JO - Current Opinion in Chemical Engineering

JF - Current Opinion in Chemical Engineering

SN - 2211-3398

IS - 1

ER -