Bioreactor design for perfusion-based, highly vascularized organ regeneration

Brent M. Bijonowski, William M. Miller*, Jason A. Wertheim

*Corresponding author for this work

Research output: Contribution to journalReview article

22 Scopus citations

Abstract

The production of bioartificial or laboratory-grown organs is a growing field centered on developing replacement organs and tissues to restore body function and providing a potential solution to the shortage of donor organs for transplantation. With the entry of engineered planar tissues, such as bladder and trachea, into clinical studies, an increasing focus is being given to designing complex, three-dimensional solid organs. As tissues become larger, thicker and more complex, the vascular network becomes crucial for supplying nutrients and maintaining viability and growth of the neo-organ. Perfusion decellularization, the process of removing cells from an entire organ, leaves the matrix of the vascular network intact. Organ engineering requires a delicate process of decellularization, sterilization, reseeding with appropriate cells, and organ maturation and stimulation to ensure optimal development. The design of bioreactors to facilitate this sequence of events has been refined to the extent that some bioartificial organs grown in these systems have been transplanted into recipient animals with sustained, though limited, function. This review focuses on the state-of-art in bioreactor development for perfusion-based bioartificial organs and highlights specific design components in need of further refinement.

Original languageEnglish (US)
Pages (from-to)32-40
Number of pages9
JournalCurrent Opinion in Chemical Engineering
Volume2
Issue number1
DOIs
StatePublished - Feb 2013

ASJC Scopus subject areas

  • Energy(all)

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