Abstract
Monitoring homeostasis is an essential aspect of obtaining pathophysiological insights for treating patients. Accurate, timely assessments of homeostatic dysregulation in deep tissues typically require expensive imaging techniques or invasive biopsies. We introduce a bioresorbable shape-adaptive materials structure that enables real-time monitoring of deep-tissue homeostasis using conventional ultrasound instruments. Collections of small bioresorbable metal disks distributed within thin, pH-responsive hydrogels, deployed by surgical implantation or syringe injection, allow ultrasound-based measurements of spatiotemporal changes in pH for early assessments of anastomotic leaks after gastrointestinal surgeries, and their bioresorption after a recovery period eliminates the need for surgical extraction. Demonstrations in small and large animal models illustrate capabilities in monitoring leakage from the small intestine, the stomach, and the pancreas.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1096-1103 |
| Number of pages | 8 |
| Journal | Science |
| Volume | 383 |
| Issue number | 6687 |
| DOIs | |
| State | Published - Mar 8 2024 |
Funding
We thank S. Li, S. Papastefan, C. Redden, and J. B. Walters for preliminary efforts in testing. We thank Y. Gao and B. Jin for helpful discussions. Histology services were provided by the Northwestern University Research Histology and Phenotyping Laboratory, which is supported by NCI P30-CA060553 awarded to the Robert H. Lurie Comprehensive Cancer Center. Metal analysis was performed at the Northwestern University Quantitative Bio-element Imaging Center. In vitro cytocompatibility was supported by the Developmental Therapeutics Core at Northwestern University and the Robert H. Lurie Comprehensive Cancer Center support grant (NCI CA060553). We thank the Veterinary Diagnostic Laboratory at the University of Illinois Urbana-Champaign for conducting blood tests. This work made use of the MatCI Facility supported by the MRSEC program of the National Science Foundation (DMR-1720139) at the Materials Research Center of Northwestern University. The schematic illustrations of the rat in Fig. 4A and the pig in Fig. 5A were created with BioRender.com. Funding: This work was funded by the Querrey-Simpson Institute for Bioelectronics (J.L., N.L., M.W., Y.Wa., R.S., Z.X., M.P., Y.Wu, J.L.C., J.G., H.L., Y.Z., T.Y., H.-Y.A., and J.A.R.); the NSF Graduate Research Fellowship (grant DGE-2234667) (J.L.C.); the Washington University School of Medicine Surgical Oncology Basic Science and Translational Research Training Program grant T32CA009621 from the National Cancer Institute (NCI) (A.H.); the Belle Carnell Regenerative Neurorehabilitation Fund (C.K.F.); the Leadership in Entrepreneurial Acceleration Program (LEAP) at Washington University in St. Louis, Missouri (Y.X., Y.Y., W.Z.R., and M.R.M.); the National Natural Science Foundation of China (grant 12272352) (H.W.); and the Emerson Collective Cancer Research Fund (C.W.H.). We thank S. Li, S. Papastefan, C. Redden, and J. B. Walters for preliminary efforts in testing. We thank Y. Gao and B. Jin for helpful discussions. Histology services were provided by the Northwestern University Research Histology and Phenotyping Laboratory, which is supported by NCI P30-CA060553 awarded to the Robert H. Lurie Comprehensive Cancer Center. Metal analysis was performed at the Northwestern University Quantitative Bio-element Imaging Center. In vitro cytocompatibility was supported by the Developmental Therapeutics Core at Northwestern University and the Robert H. Lurie Comprehensive Cancer Center support grant (NCI CA060553). We thank the Veterinary Diagnostic Laboratory at the University of Illinois Urbana-Champaign for conducting blood tests. This work made use of the MatCI Facility supported by the MRSEC program of the National Science Foundation (DMR-1720139) at the Materials Research Center of Northwestern University. The schematic illustrations of the rat in Fig. 4A and the pig in Fig. 5A were created with BioRender.com. Funding: This work was funded by the Querrey-Simpson Institute for Bioelectronics (J.L., N.L., M.W., Y.Wa., R.S., Z.X., M.P., Y.Wu, J.L.C., J.G., H.L., Y.Z., T.Y., H.-Y.A., and J.A.R.); the NSF Graduate Research Fellowship (grant DGE-2234667) (J.L.C.); the Washington University School of Medicine Surgical Oncology Basic Science and Translational Research Training Program grant T32CA009621 from the National Cancer Institute (NCI) (A.H.); the Belle Carnell Regenerative Neurorehabilitation Fund (C.K.F.); the Leadership in Entrepreneurial Acceleration Program (LEAP) at Washington University in St. Louis, Missouri (Y.X., Y.Y., W.Z.R., and M.R.M.); the National Natural Science Foundation of China (grant 12272352) (H.W.); and the Emerson Collective Cancer Research Fund (C.W.H.). Author contributions: Conceptualization: J.L., N.L., C.W.H., and J.A.R. Methodology: J.L., N.L., Y.X., M.W., Y.Y., C.W.H., and J.A.R. Theoretical simulations: H.Z., S.L., Y.H., and H.W. Investigation: J.L., N.L., Y.X., M.W., Y.Y., A.H., R.S., Z.X., M.P., Y.Wu, J.L.C., J.G., H.L., Y.Z., T.Y., H.-Y.A., W.Z.R., C.K.F., M.R.M., and C.W.H. Software: Y.Wa. Data analysis: J.L., N.L., Y.X., and M.W. Visualization: J.L., N.L., Y.X., and M.W. Supervision: C.W.H., H.W., and J.A.R. Writing – original draft: J.L., N.L., Y.X., M.W., H.Z., H.W., and J.A.R. Writing – review & editing: J.L., N.L., Y.X., M.W., H.Z., Y.H., C.W.H., H.W., and J.A.R. Competing interests: The authors declare that they have no competing interests. Data and materials availability: All data are available in the manuscript or the supplementary materials. License information: Copyright © 2024 the authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original US government works. https://www.science.org/about/science-licenses-journal-article-reuse
ASJC Scopus subject areas
- General