Abstract
The glycosyltransferase activities involved in the biosynthesis in vitro of neutral blood group-related glycosphingolipids were measured in African green monkey kidney cells (Vero) grown in culture. The a-fucosyltransferases which catalyzed the reaction between GDP-fucose and corresponding acceptors to form H-active and novel Lea-type glycosphingolipids were characterized in membrane fractions isolated from Vero cells and monkey bone marrow. Using 125I-labeled Ulex europeus and Lotus tetragonolobus lectins the differential binding to Vero cell surface glycoproteins and glycolipids was studied under various conditions.
Original language | English (US) |
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Pages (from-to) | 1380-1388 |
Number of pages | 9 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 66 |
Issue number | 4 |
DOIs | |
State | Published - Oct 27 1975 |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology