Biosynthesis in vitro and the pattern of lectin binding receptors in monkey kidney cell surfaces

Manju Basu*, Joseph R. Moskal, David A. Gardner, Subhash Basu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

The glycosyltransferase activities involved in the biosynthesis in vitro of neutral blood group-related glycosphingolipids were measured in African green monkey kidney cells (Vero) grown in culture. The a-fucosyltransferases which catalyzed the reaction between GDP-fucose and corresponding acceptors to form H-active and novel Lea-type glycosphingolipids were characterized in membrane fractions isolated from Vero cells and monkey bone marrow. Using 125I-labeled Ulex europeus and Lotus tetragonolobus lectins the differential binding to Vero cell surface glycoproteins and glycolipids was studied under various conditions.

Original languageEnglish (US)
Pages (from-to)1380-1388
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume66
Issue number4
DOIs
StatePublished - Oct 27 1975

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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