The glycosyltransferase activities involved in the biosynthesis in vitro of neutral blood group-related glycosphingolipids were measured in African green monkey kidney cells (Vero) grown in culture. The a-fucosyltransferases which catalyzed the reaction between GDP-fucose and corresponding acceptors to form H-active and novel Lea-type glycosphingolipids were characterized in membrane fractions isolated from Vero cells and monkey bone marrow. Using 125I-labeled Ulex europeus and Lotus tetragonolobus lectins the differential binding to Vero cell surface glycoproteins and glycolipids was studied under various conditions.
|Original language||English (US)|
|Number of pages||9|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Oct 27 1975|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology