Biosynthesis in vitro and the pattern of lectin binding receptors in monkey kidney cell surfaces

Manju Basu; Joseph R. Moskal; David A. Gardner; Subhash Basu

doi:10.1016/0006-291X(75)90512-4

Biosynthesis in vitro and the pattern of lectin binding receptors in monkey kidney cell surfaces

Manju Basu^*, Joseph R. Moskal, David A. Gardner, Subhash Basu

^*Corresponding author for this work

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The glycosyltransferase activities involved in the biosynthesis in vitro of neutral blood group-related glycosphingolipids were measured in African green monkey kidney cells (Vero) grown in culture. The a-fucosyltransferases which catalyzed the reaction between GDP-fucose and corresponding acceptors to form H-active and novel Le^a-type glycosphingolipids were characterized in membrane fractions isolated from Vero cells and monkey bone marrow. Using ¹²⁵I-labeled Ulex europeus and Lotus tetragonolobus lectins the differential binding to Vero cell surface glycoproteins and glycolipids was studied under various conditions.

Original language	English (US)
Pages (from-to)	1380-1388
Number of pages	9
Journal	Biochemical and Biophysical Research Communications
Volume	66
Issue number	4
DOIs	https://doi.org/10.1016/0006-291X(75)90512-4
State	Published - Oct 27 1975

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(75)90512-4

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title = "Biosynthesis in vitro and the pattern of lectin binding receptors in monkey kidney cell surfaces",

abstract = "The glycosyltransferase activities involved in the biosynthesis in vitro of neutral blood group-related glycosphingolipids were measured in African green monkey kidney cells (Vero) grown in culture. The a-fucosyltransferases which catalyzed the reaction between GDP-fucose and corresponding acceptors to form H-active and novel Lea-type glycosphingolipids were characterized in membrane fractions isolated from Vero cells and monkey bone marrow. Using 125I-labeled Ulex europeus and Lotus tetragonolobus lectins the differential binding to Vero cell surface glycoproteins and glycolipids was studied under various conditions.",

author = "Manju Basu and Moskal, {Joseph R.} and Gardner, {David A.} and Subhash Basu",

year = "1975",

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T1 - Biosynthesis in vitro and the pattern of lectin binding receptors in monkey kidney cell surfaces

AU - Basu, Manju

AU - Moskal, Joseph R.

AU - Gardner, David A.

AU - Basu, Subhash

PY - 1975/10/27

Y1 - 1975/10/27

N2 - The glycosyltransferase activities involved in the biosynthesis in vitro of neutral blood group-related glycosphingolipids were measured in African green monkey kidney cells (Vero) grown in culture. The a-fucosyltransferases which catalyzed the reaction between GDP-fucose and corresponding acceptors to form H-active and novel Lea-type glycosphingolipids were characterized in membrane fractions isolated from Vero cells and monkey bone marrow. Using 125I-labeled Ulex europeus and Lotus tetragonolobus lectins the differential binding to Vero cell surface glycoproteins and glycolipids was studied under various conditions.

AB - The glycosyltransferase activities involved in the biosynthesis in vitro of neutral blood group-related glycosphingolipids were measured in African green monkey kidney cells (Vero) grown in culture. The a-fucosyltransferases which catalyzed the reaction between GDP-fucose and corresponding acceptors to form H-active and novel Lea-type glycosphingolipids were characterized in membrane fractions isolated from Vero cells and monkey bone marrow. Using 125I-labeled Ulex europeus and Lotus tetragonolobus lectins the differential binding to Vero cell surface glycoproteins and glycolipids was studied under various conditions.

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

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ER -

Biosynthesis in vitro and the pattern of lectin binding receptors in monkey kidney cell surfaces

Abstract

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