Biosynthesis of globoside and Forssman-related glycosphingolipid in mouse adrenal Y-1 tumor cells

Kwok Kam Yeung*, Joseph R. Moskal, Jao Long Chien, David A. Gardner, Subhash Basu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

The activities of four glycolipid glycosyltransferases involved in the biosynthesis in vitro of globoside and Forssman hapten were measured in normal mouse adrenal tissues, Y-1 mouse adrenal tumor cells, and tumors derived therefrom. These enzyme activities, found in Golgi-rich membranes isolated on a sucrose density gradient, were higher in the tumor cell cultures than in normal mouse adrenal tissue. The ratio between long chain oligoglycosylceramides and short chain glycosphingolipids was higher in the case of Y-l-K cells treated with dibutyryl cAMP than in concanavalin A, colchicine treated, or untreated control cells in culture.

Original languageEnglish (US)
Pages (from-to)252-260
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume59
Issue number1
DOIs
StatePublished - Jul 10 1974

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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