Biosynthesis of globoside and Forssman-related glycosphingolipid in mouse adrenal Y-1 tumor cells

Kwok Kam Yeung; Joseph R. Moskal; Jao Long Chien; David A. Gardner; Subhash Basu

doi:10.1016/S0006-291X(74)80200-7

Biosynthesis of globoside and Forssman-related glycosphingolipid in mouse adrenal Y-1 tumor cells

Kwok Kam Yeung^*, Joseph R. Moskal, Jao Long Chien, David A. Gardner, Subhash Basu

^*Corresponding author for this work

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

The activities of four glycolipid glycosyltransferases involved in the biosynthesis in vitro of globoside and Forssman hapten were measured in normal mouse adrenal tissues, Y-1 mouse adrenal tumor cells, and tumors derived therefrom. These enzyme activities, found in Golgi-rich membranes isolated on a sucrose density gradient, were higher in the tumor cell cultures than in normal mouse adrenal tissue. The ratio between long chain oligoglycosylceramides and short chain glycosphingolipids was higher in the case of Y-l-K cells treated with dibutyryl cAMP than in concanavalin A, colchicine treated, or untreated control cells in culture.

Original language	English (US)
Pages (from-to)	252-260
Number of pages	9
Journal	Biochemical and Biophysical Research Communications
Volume	59
Issue number	1
DOIs	https://doi.org/10.1016/S0006-291X(74)80200-7
State	Published - Jul 10 1974

Funding

This investigation was supported by NIH Research Grant NS-09541-04 and a grant-in-aid from Miles Laboratories, Inc., Elkhart, Indiana, to S.B. + Predoctoral fellows in the Chemistry Department of the Univ. of Notre Dame, supported in part by the grant NS-O9541 from the NIH.

ASJC Scopus subject areas

Molecular Biology
Biophysics
Biochemistry
Cell Biology

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10.1016/S0006-291X(74)80200-7

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title = "Biosynthesis of globoside and Forssman-related glycosphingolipid in mouse adrenal Y-1 tumor cells",

abstract = "The activities of four glycolipid glycosyltransferases involved in the biosynthesis in vitro of globoside and Forssman hapten were measured in normal mouse adrenal tissues, Y-1 mouse adrenal tumor cells, and tumors derived therefrom. These enzyme activities, found in Golgi-rich membranes isolated on a sucrose density gradient, were higher in the tumor cell cultures than in normal mouse adrenal tissue. The ratio between long chain oligoglycosylceramides and short chain glycosphingolipids was higher in the case of Y-l-K cells treated with dibutyryl cAMP than in concanavalin A, colchicine treated, or untreated control cells in culture.",

author = "Yeung, {Kwok Kam} and Moskal, {Joseph R.} and Chien, {Jao Long} and Gardner, {David A.} and Subhash Basu",

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AU - Chien, Jao Long

AU - Gardner, David A.

AU - Basu, Subhash

N1 - Funding Information: This investigation was supported by NIH Research Grant NS-09541-04 and a grant-in-aid from Miles Laboratories, Inc., Elkhart, Indiana, to S.B. + Predoctoral fellows in the Chemistry Department of the Univ. of Notre Dame, supported in part by the grant NS-O9541 from the NIH.

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AB - The activities of four glycolipid glycosyltransferases involved in the biosynthesis in vitro of globoside and Forssman hapten were measured in normal mouse adrenal tissues, Y-1 mouse adrenal tumor cells, and tumors derived therefrom. These enzyme activities, found in Golgi-rich membranes isolated on a sucrose density gradient, were higher in the tumor cell cultures than in normal mouse adrenal tissue. The ratio between long chain oligoglycosylceramides and short chain glycosphingolipids was higher in the case of Y-l-K cells treated with dibutyryl cAMP than in concanavalin A, colchicine treated, or untreated control cells in culture.

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Biosynthesis of globoside and Forssman-related glycosphingolipid in mouse adrenal Y-1 tumor cells

Abstract

Funding

ASJC Scopus subject areas

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