Biosynthesis of human papillomavirus from a continuous cell line upon epithelial differentiation

Craig Meyers, Mark G. Frattini, John B. Hudson, Laimonis A. Laimins*

*Corresponding author for this work

Research output: Contribution to journalArticle

339 Scopus citations

Abstract

The study of the human pathogen papillomaviruses (HPVs) has been hampered by the inability to propagate the virus in tissue culture. The addition of 12-O-tetradecanoyl phorbol-13-acetate to the media of organotypic (raft) cultures increased expression of physiological markers of keratinocyte differentiation and concomitantly induced production of virions. Capsid production was detected in differentiated suprabasal cells. Virions approximately 54 nanometers in size were observed by electron microscopy in raft tissue cross sections in the suprabasal layers. Virions purified through isopycnic gradients were found to contain type 31b DNA and exhibited an icosahedral shape similar to that of papillomaviruses found in clinical samples.

Original languageEnglish (US)
Pages (from-to)971-973
Number of pages3
JournalScience
Volume257
Issue number5072
DOIs
StatePublished - Jan 1 1992

ASJC Scopus subject areas

  • General

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