Biosynthesis of human papillomavirus from a continuous cell line upon epithelial differentiation

Craig Meyers; Mark G. Frattini; John B. Hudson; Laimonis A. Laimins

doi:10.1126/science.1323879

Biosynthesis of human papillomavirus from a continuous cell line upon epithelial differentiation

Craig Meyers, Mark G. Frattini, John B. Hudson, Laimonis A. Laimins^*

^*Corresponding author for this work

Microbiology-Immunology

Research output: Contribution to journal › Article › peer-review

198 Scopus citations

Abstract

The study of the human pathogen papillomaviruses (HPVs) has been hampered by the inability to propagate the virus in tissue culture. The addition of 12-O-tetradecanoyl phorbol-13-acetate to the media of organotypic (raft) cultures increased expression of physiological markers of keratinocyte differentiation and concomitantly induced production of virions. Capsid production was detected in differentiated suprabasal cells. Virions approximately 54 nanometers in size were observed by electron microscopy in raft tissue cross sections in the suprabasal layers. Virions purified through isopycnic gradients were found to contain type 31b DNA and exhibited an icosahedral shape similar to that of papillomaviruses found in clinical samples.

Original language	English (US)
Pages (from-to)	971-973
Number of pages	3
Journal	Science
Volume	257
Issue number	5072
DOIs	https://doi.org/10.1126/science.1323879
State	Published - 1992

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title = "Biosynthesis of human papillomavirus from a continuous cell line upon epithelial differentiation",

abstract = "The study of the human pathogen papillomaviruses (HPVs) has been hampered by the inability to propagate the virus in tissue culture. The addition of 12-O-tetradecanoyl phorbol-13-acetate to the media of organotypic (raft) cultures increased expression of physiological markers of keratinocyte differentiation and concomitantly induced production of virions. Capsid production was detected in differentiated suprabasal cells. Virions approximately 54 nanometers in size were observed by electron microscopy in raft tissue cross sections in the suprabasal layers. Virions purified through isopycnic gradients were found to contain type 31b DNA and exhibited an icosahedral shape similar to that of papillomaviruses found in clinical samples.",

author = "Craig Meyers and Frattini, {Mark G.} and Hudson, {John B.} and Laimins, {Laimonis A.}",

year = "1992",

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AU - Frattini, Mark G.

AU - Hudson, John B.

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AB - The study of the human pathogen papillomaviruses (HPVs) has been hampered by the inability to propagate the virus in tissue culture. The addition of 12-O-tetradecanoyl phorbol-13-acetate to the media of organotypic (raft) cultures increased expression of physiological markers of keratinocyte differentiation and concomitantly induced production of virions. Capsid production was detected in differentiated suprabasal cells. Virions approximately 54 nanometers in size were observed by electron microscopy in raft tissue cross sections in the suprabasal layers. Virions purified through isopycnic gradients were found to contain type 31b DNA and exhibited an icosahedral shape similar to that of papillomaviruses found in clinical samples.

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Biosynthesis of human papillomavirus from a continuous cell line upon epithelial differentiation

Abstract

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