Biotin-dextran: a sensitive anterograde tracer for neuroanatomic studies in rat and monkey

Heike M. Brandt, A. Vania Apkarian*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

200 Scopus citations

Abstract

The properties of a new anterograde tracer, biotin-dextran (BD), are reported. Iontophoretic or pressure injections of BD in the spinal cord of rats and monkeys revealed terminal-like BD label in many regions of the brain. The BD label was visualized by an avidin-biotin reaction combined with diaminobenzidine, with and without nickel enhancement. This reaction resulted in permanent label and revealed the fine morphology of terminal boutons and en-passant endings. Biotin-dextran is an excellent anterograde tracer that can also be visualized at the electron microscopic level, used in combination with other retrograde and anterograde tracers and with immunohistochemical labeling of neurotransmitters.

Original languageEnglish (US)
Pages (from-to)35-40
Number of pages6
JournalJournal of Neuroscience Methods
Volume45
Issue number1-2
DOIs
StatePublished - 1992

Keywords

  • Axonal label
  • Biotin
  • Dextran
  • Diaminobenzidine
  • Retrograde label
  • Spinal cord

ASJC Scopus subject areas

  • Neuroscience(all)

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