Biotin-dextran: a sensitive anterograde tracer for neuroanatomic studies in rat and monkey

Heike M. Brandt; A. Vania Apkarian

doi:10.1016/0165-0270(92)90041-B

Biotin-dextran: a sensitive anterograde tracer for neuroanatomic studies in rat and monkey

Heike M. Brandt, A. Vania Apkarian^*

^*Corresponding author for this work

Neuroscience

Research output: Contribution to journal › Article › peer-review

205 Scopus citations

Abstract

The properties of a new anterograde tracer, biotin-dextran (BD), are reported. Iontophoretic or pressure injections of BD in the spinal cord of rats and monkeys revealed terminal-like BD label in many regions of the brain. The BD label was visualized by an avidin-biotin reaction combined with diaminobenzidine, with and without nickel enhancement. This reaction resulted in permanent label and revealed the fine morphology of terminal boutons and en-passant endings. Biotin-dextran is an excellent anterograde tracer that can also be visualized at the electron microscopic level, used in combination with other retrograde and anterograde tracers and with immunohistochemical labeling of neurotransmitters.

Original language	English (US)
Pages (from-to)	35-40
Number of pages	6
Journal	Journal of Neuroscience Methods
Volume	45
Issue number	1-2
DOIs	https://doi.org/10.1016/0165-0270(92)90041-B
State	Published - 1992

Keywords

Axonal label
Biotin
Dextran
Diaminobenzidine
Retrograde label
Spinal cord

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/0165-0270(92)90041-B

Cite this

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title = "Biotin-dextran: a sensitive anterograde tracer for neuroanatomic studies in rat and monkey",

abstract = "The properties of a new anterograde tracer, biotin-dextran (BD), are reported. Iontophoretic or pressure injections of BD in the spinal cord of rats and monkeys revealed terminal-like BD label in many regions of the brain. The BD label was visualized by an avidin-biotin reaction combined with diaminobenzidine, with and without nickel enhancement. This reaction resulted in permanent label and revealed the fine morphology of terminal boutons and en-passant endings. Biotin-dextran is an excellent anterograde tracer that can also be visualized at the electron microscopic level, used in combination with other retrograde and anterograde tracers and with immunohistochemical labeling of neurotransmitters.",

keywords = "Axonal label, Biotin, Dextran, Diaminobenzidine, Retrograde label, Spinal cord",

author = "Brandt, {Heike M.} and Apkarian, {A. Vania}",

note = "Funding Information: We are grateful to Richard T. Stevens for his constructive criticism of the manuscript. We also thank Rick Lin for his helpful advice. This study was supported by the Department of Neurosurgery and the Perkins Foundation.",

year = "1992",

doi = "10.1016/0165-0270(92)90041-B",

language = "English (US)",

volume = "45",

pages = "35--40",

journal = "Journal of Neuroscience Methods",

issn = "0165-0270",

publisher = "Elsevier",

number = "1-2",

}

TY - JOUR

T1 - Biotin-dextran

T2 - a sensitive anterograde tracer for neuroanatomic studies in rat and monkey

AU - Brandt, Heike M.

AU - Apkarian, A. Vania

N1 - Funding Information: We are grateful to Richard T. Stevens for his constructive criticism of the manuscript. We also thank Rick Lin for his helpful advice. This study was supported by the Department of Neurosurgery and the Perkins Foundation.

PY - 1992

Y1 - 1992

N2 - The properties of a new anterograde tracer, biotin-dextran (BD), are reported. Iontophoretic or pressure injections of BD in the spinal cord of rats and monkeys revealed terminal-like BD label in many regions of the brain. The BD label was visualized by an avidin-biotin reaction combined with diaminobenzidine, with and without nickel enhancement. This reaction resulted in permanent label and revealed the fine morphology of terminal boutons and en-passant endings. Biotin-dextran is an excellent anterograde tracer that can also be visualized at the electron microscopic level, used in combination with other retrograde and anterograde tracers and with immunohistochemical labeling of neurotransmitters.

AB - The properties of a new anterograde tracer, biotin-dextran (BD), are reported. Iontophoretic or pressure injections of BD in the spinal cord of rats and monkeys revealed terminal-like BD label in many regions of the brain. The BD label was visualized by an avidin-biotin reaction combined with diaminobenzidine, with and without nickel enhancement. This reaction resulted in permanent label and revealed the fine morphology of terminal boutons and en-passant endings. Biotin-dextran is an excellent anterograde tracer that can also be visualized at the electron microscopic level, used in combination with other retrograde and anterograde tracers and with immunohistochemical labeling of neurotransmitters.

KW - Axonal label

KW - Biotin

KW - Dextran

KW - Diaminobenzidine

KW - Retrograde label

KW - Spinal cord

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U2 - 10.1016/0165-0270(92)90041-B

DO - 10.1016/0165-0270(92)90041-B

M3 - Article

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AN - SCOPUS:0026443162

SN - 0165-0270

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JO - Journal of Neuroscience Methods

JF - Journal of Neuroscience Methods

IS - 1-2

ER -

Biotin-dextran: a sensitive anterograde tracer for neuroanatomic studies in rat and monkey

Abstract

Keywords

ASJC Scopus subject areas

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