Biotin uptake in cultured hepatocytes from normal and biotin-deficient rats

Debrah Weiner*, Barry Wolf

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


Biotin uptake was studied in isolated cultured hepatocytes of normal and biotin-deficient rats. Biotin uptake was temperature-dependent with respect to physical, but not to chemical, processes, proportional to the exogenous biotin concentration in the medium, independent of pH and sodium ion concentrations of the medium, and uneffected by the presence of structural analogues of biotin or metabolic inhibitors in both normal and biotin-deficient hepatocytes. These results suggest that biotin uptake occurs by a passive, nonmediated, non-energy-dependent mechanism in rat hepatocytes.

Original languageEnglish (US)
Pages (from-to)271-281
Number of pages11
JournalBiochemical Medicine and Metabolic Biology
Issue number3
StatePublished - Dec 1990

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry


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