Birefringence Measurement of Rapid Structural Changes during Collagen Denaturation

Vanitha Sankaran; Joseph T. Walsh

doi:10.1111/j.1751-1097.1998.tb05294.x

Birefringence Measurement of Rapid Structural Changes during Collagen Denaturation

Vanitha Sankaran, Joseph T. Walsh^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

Linear birefringence, an optical property that results from a material's structure and composition, can be used to study dynamic changes in tissue structure. Single, 200 μs-long pulses from a Ho:YAG laser emitting 2.1 μm radiation were used to induce changes in the linear birefringence of rat tail tendon. Such changes were measured on a millisecond timescale. The measured rate coefficients describing the denaturation are not predicted by previous studies of collagen denaturation induced by slower, lower-temperature heating. Two types of laser-induced collagen denaturation can be differentiated: thermal denaturation, which appears rate-limited, and thermomechanical denaturation, which is observed at higher laser radiant exposures. Neither process is described by standard Arrhenius-type kinetic models.

Original language	English (US)
Pages (from-to)	846-851
Number of pages	6
Journal	Photochemistry and Photobiology
Volume	68
Issue number	6
DOIs	https://doi.org/10.1111/j.1751-1097.1998.tb05294.x
State	Published - Dec 1998
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Physical and Theoretical Chemistry

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10.1111/j.1751-1097.1998.tb05294.x

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title = "Birefringence Measurement of Rapid Structural Changes during Collagen Denaturation",

abstract = "Linear birefringence, an optical property that results from a material's structure and composition, can be used to study dynamic changes in tissue structure. Single, 200 μs-long pulses from a Ho:YAG laser emitting 2.1 μm radiation were used to induce changes in the linear birefringence of rat tail tendon. Such changes were measured on a millisecond timescale. The measured rate coefficients describing the denaturation are not predicted by previous studies of collagen denaturation induced by slower, lower-temperature heating. Two types of laser-induced collagen denaturation can be differentiated: thermal denaturation, which appears rate-limited, and thermomechanical denaturation, which is observed at higher laser radiant exposures. Neither process is described by standard Arrhenius-type kinetic models.",

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AB - Linear birefringence, an optical property that results from a material's structure and composition, can be used to study dynamic changes in tissue structure. Single, 200 μs-long pulses from a Ho:YAG laser emitting 2.1 μm radiation were used to induce changes in the linear birefringence of rat tail tendon. Such changes were measured on a millisecond timescale. The measured rate coefficients describing the denaturation are not predicted by previous studies of collagen denaturation induced by slower, lower-temperature heating. Two types of laser-induced collagen denaturation can be differentiated: thermal denaturation, which appears rate-limited, and thermomechanical denaturation, which is observed at higher laser radiant exposures. Neither process is described by standard Arrhenius-type kinetic models.

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Birefringence Measurement of Rapid Structural Changes during Collagen Denaturation

Abstract

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