Blood leukocyte Alu and LINE-1 methylation and gastric cancer risk in the Shanghai Women's Health Study

Y. Gao; A. Baccarelli; X. O. Shu; B. T. Ji; K. Yu; L. Tarantini; G. Yang; H. L. Li; L. Hou; N. Rothman; W. Zheng; Y. T. Gao; W. H. Chow

doi:10.1038/bjc.2011.562

Blood leukocyte Alu and LINE-1 methylation and gastric cancer risk in the Shanghai Women's Health Study

Y. Gao^*, A. Baccarelli, X. O. Shu, B. T. Ji, K. Yu, L. Tarantini, G. Yang, H. L. Li, L. Hou, N. Rothman, W. Zheng, Y. T. Gao, W. H. Chow

^*Corresponding author for this work

Preventive Medicine

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

Background: Recent data suggest a link between blood leukocyte DNA methylation, and cancer risk. However, reports on DNA methylation from a prospective study are unavailable for gastric cancer.Methods: We explored the association between methylation in pre-diagnostic blood leukocyte DNA and gastric cancer risk in a case-control study nested in the prospective Shanghai Women's Health Study cohort. Incident gastric cancer cases (n=192) and matched controls (n=384) were included in the study. Methylation of Alu and long interspersed nucleotide elements (LINE)-1 were evaluated using bisulphite pyrosequencing. Odds ratios (ORs) and 95% confidence intervals (CI) were calculated from logistic regression adjusting for potential confounders. Results: Alu methylation was inversely associated with gastric cancer risk, mainly among cases diagnosed one or more years after blood collection. After excluding cases diagnosed during the first year of follow-up, the ORs for the third, second, and first quartiles of Alu methylation compared with the highest quartile were 2.43 (1.43-4.13), 1.47(0.85-2.57), and 2.22 (1.28-3.84), respectively. This association appeared to be modified by dietary intake, particularly isoflavone. In contrast, LINE-1 methylation levels were not associated with gastric cancer risk. Conclusion: Evidence from this prospective study is consistent with the hypothesis that DNA hypomethylation in blood leukocytes may be related to cancer risk, including risk of gastric cancer.

Original language	English (US)
Pages (from-to)	585-591
Number of pages	7
Journal	British Journal of Cancer
Volume	106
Issue number	3
DOIs	https://doi.org/10.1038/bjc.2011.562
State	Published - Jan 31 2012

Funding

This study was supported by the Intramural Research Program of the Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health. Dr Baccarelli is partially supported by New Investigator funding from NIEHS (ES00002). Finally, we acknowledge the study participants for donating their time and making this study possible.

Keywords

DNA methylation
Gastric cancer
Leukocyte

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1038/bjc.2011.562

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title = "Blood leukocyte Alu and LINE-1 methylation and gastric cancer risk in the Shanghai Women's Health Study",

abstract = "Background: Recent data suggest a link between blood leukocyte DNA methylation, and cancer risk. However, reports on DNA methylation from a prospective study are unavailable for gastric cancer.Methods: We explored the association between methylation in pre-diagnostic blood leukocyte DNA and gastric cancer risk in a case-control study nested in the prospective Shanghai Women's Health Study cohort. Incident gastric cancer cases (n=192) and matched controls (n=384) were included in the study. Methylation of Alu and long interspersed nucleotide elements (LINE)-1 were evaluated using bisulphite pyrosequencing. Odds ratios (ORs) and 95% confidence intervals (CI) were calculated from logistic regression adjusting for potential confounders. Results: Alu methylation was inversely associated with gastric cancer risk, mainly among cases diagnosed one or more years after blood collection. After excluding cases diagnosed during the first year of follow-up, the ORs for the third, second, and first quartiles of Alu methylation compared with the highest quartile were 2.43 (1.43-4.13), 1.47(0.85-2.57), and 2.22 (1.28-3.84), respectively. This association appeared to be modified by dietary intake, particularly isoflavone. In contrast, LINE-1 methylation levels were not associated with gastric cancer risk. Conclusion: Evidence from this prospective study is consistent with the hypothesis that DNA hypomethylation in blood leukocytes may be related to cancer risk, including risk of gastric cancer.",

keywords = "DNA methylation, Gastric cancer, Leukocyte",

author = "Y. Gao and A. Baccarelli and Shu, {X. O.} and Ji, {B. T.} and K. Yu and L. Tarantini and G. Yang and Li, {H. L.} and L. Hou and N. Rothman and W. Zheng and Gao, {Y. T.} and Chow, {W. H.}",

note = "Funding Information: This study was supported by the Intramural Research Program of the Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health. Dr Baccarelli is partially supported by New Investigator funding from NIEHS (ES00002). Finally, we acknowledge the study participants for donating their time and making this study possible.",

year = "2012",

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day = "31",

doi = "10.1038/bjc.2011.562",

language = "English (US)",

volume = "106",

pages = "585--591",

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T1 - Blood leukocyte Alu and LINE-1 methylation and gastric cancer risk in the Shanghai Women's Health Study

AU - Gao, Y.

AU - Baccarelli, A.

AU - Shu, X. O.

AU - Ji, B. T.

AU - Yu, K.

AU - Tarantini, L.

AU - Yang, G.

AU - Li, H. L.

AU - Hou, L.

AU - Rothman, N.

AU - Zheng, W.

AU - Gao, Y. T.

AU - Chow, W. H.

N1 - Funding Information: This study was supported by the Intramural Research Program of the Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health. Dr Baccarelli is partially supported by New Investigator funding from NIEHS (ES00002). Finally, we acknowledge the study participants for donating their time and making this study possible.

PY - 2012/1/31

Y1 - 2012/1/31

N2 - Background: Recent data suggest a link between blood leukocyte DNA methylation, and cancer risk. However, reports on DNA methylation from a prospective study are unavailable for gastric cancer.Methods: We explored the association between methylation in pre-diagnostic blood leukocyte DNA and gastric cancer risk in a case-control study nested in the prospective Shanghai Women's Health Study cohort. Incident gastric cancer cases (n=192) and matched controls (n=384) were included in the study. Methylation of Alu and long interspersed nucleotide elements (LINE)-1 were evaluated using bisulphite pyrosequencing. Odds ratios (ORs) and 95% confidence intervals (CI) were calculated from logistic regression adjusting for potential confounders. Results: Alu methylation was inversely associated with gastric cancer risk, mainly among cases diagnosed one or more years after blood collection. After excluding cases diagnosed during the first year of follow-up, the ORs for the third, second, and first quartiles of Alu methylation compared with the highest quartile were 2.43 (1.43-4.13), 1.47(0.85-2.57), and 2.22 (1.28-3.84), respectively. This association appeared to be modified by dietary intake, particularly isoflavone. In contrast, LINE-1 methylation levels were not associated with gastric cancer risk. Conclusion: Evidence from this prospective study is consistent with the hypothesis that DNA hypomethylation in blood leukocytes may be related to cancer risk, including risk of gastric cancer.

AB - Background: Recent data suggest a link between blood leukocyte DNA methylation, and cancer risk. However, reports on DNA methylation from a prospective study are unavailable for gastric cancer.Methods: We explored the association between methylation in pre-diagnostic blood leukocyte DNA and gastric cancer risk in a case-control study nested in the prospective Shanghai Women's Health Study cohort. Incident gastric cancer cases (n=192) and matched controls (n=384) were included in the study. Methylation of Alu and long interspersed nucleotide elements (LINE)-1 were evaluated using bisulphite pyrosequencing. Odds ratios (ORs) and 95% confidence intervals (CI) were calculated from logistic regression adjusting for potential confounders. Results: Alu methylation was inversely associated with gastric cancer risk, mainly among cases diagnosed one or more years after blood collection. After excluding cases diagnosed during the first year of follow-up, the ORs for the third, second, and first quartiles of Alu methylation compared with the highest quartile were 2.43 (1.43-4.13), 1.47(0.85-2.57), and 2.22 (1.28-3.84), respectively. This association appeared to be modified by dietary intake, particularly isoflavone. In contrast, LINE-1 methylation levels were not associated with gastric cancer risk. Conclusion: Evidence from this prospective study is consistent with the hypothesis that DNA hypomethylation in blood leukocytes may be related to cancer risk, including risk of gastric cancer.

KW - DNA methylation

KW - Gastric cancer

KW - Leukocyte

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DO - 10.1038/bjc.2011.562

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JF - British Journal of Cancer

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Blood leukocyte Alu and LINE-1 methylation and gastric cancer risk in the Shanghai Women's Health Study

Abstract

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

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