Blood pressure and expression of microRNAs in whole blood

Zhou Zhang, Brian Thomas Joyce, Jacob K. Kresovich, Yinan Zheng, Jia Zhong, Ruchi Patel, Wei Zhang, Lei Liu, Chang Dou, John P. McCracken, Anaiteâ DõÂaz, Valeria Motta, Marco Sanchez-Guerra, Shurui Bian, Pier Alberto Bertazzi, Joel Schwartz, Andrea A. Baccarelli, Sheng Wang, Lifang Hou

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background Blood pressure (BP) is a complex, multifactorial clinical outcome driven by genetic susceptibility, behavioral choices, and environmental factors. Many molecular mechanisms have been proposed for the pathophysiology of high BP even as its prevalence continues to grow worldwide, increasing morbidity and marking it as a major public health concern. To address this, we evaluated miRNA profiling in blood leukocytes as potential biomarkers of BP and BP-related risk factors. Methods The Beijing Truck Driver Air Pollution Study included 60 truck drivers and 60 office workers examined in 2008. On two days separated by 1-2 weeks, we examined three BP measures: systolic, diastolic, and mean arterial pressure measured at both pre-and post-work exams for blood NanoString nCounter miRNA profiles. We used covariate-Adjusted linear mixedeffect models to examine associations between BP and increased miRNA expression in both pooled and risk factor-stratified analyses. Results Overall 43 miRNAs were associated with pre-work BP (FDR<0.05). In stratified analyses different but overlapping groups of miRNAs were associated with pre-work BP in truck drivershigh-BMI participants, and usual alcohol drinkers (FDR<0.05). Only four miRNAs were associated with post-work BP (FDR<0.05), in ever smokers. Conclusion Our results suggest that many miRNAs were significantly associated with BP in subgroups exposed to known hypertension risk factors. These findings shed light on the underlying molecular mechanisms of BP, and may assist with the development of a miRNA panel for early detection of hypertension.

Original languageEnglish (US)
Article numbere0173550
JournalPloS one
Volume12
Issue number3
DOIs
StatePublished - Mar 1 2017

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Blood pressure
MicroRNAs
microRNA
blood pressure
Blood
Blood Pressure
blood
trucks
Motor Vehicles
hypertension
Truck drivers
risk factors
Hypertension
Air Pollution
air pollution
Genetic Predisposition to Disease
pathophysiology
Biomarkers
Public health
Air pollution

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Zhang, Z., Joyce, B. T., Kresovich, J. K., Zheng, Y., Zhong, J., Patel, R., ... Hou, L. (2017). Blood pressure and expression of microRNAs in whole blood. PloS one, 12(3), [e0173550]. https://doi.org/10.1371/journal.pone.0173550
Zhang, Zhou ; Joyce, Brian Thomas ; Kresovich, Jacob K. ; Zheng, Yinan ; Zhong, Jia ; Patel, Ruchi ; Zhang, Wei ; Liu, Lei ; Dou, Chang ; McCracken, John P. ; DõÂaz, Anaiteâ ; Motta, Valeria ; Sanchez-Guerra, Marco ; Bian, Shurui ; Bertazzi, Pier Alberto ; Schwartz, Joel ; Baccarelli, Andrea A. ; Wang, Sheng ; Hou, Lifang. / Blood pressure and expression of microRNAs in whole blood. In: PloS one. 2017 ; Vol. 12, No. 3.
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abstract = "Background Blood pressure (BP) is a complex, multifactorial clinical outcome driven by genetic susceptibility, behavioral choices, and environmental factors. Many molecular mechanisms have been proposed for the pathophysiology of high BP even as its prevalence continues to grow worldwide, increasing morbidity and marking it as a major public health concern. To address this, we evaluated miRNA profiling in blood leukocytes as potential biomarkers of BP and BP-related risk factors. Methods The Beijing Truck Driver Air Pollution Study included 60 truck drivers and 60 office workers examined in 2008. On two days separated by 1-2 weeks, we examined three BP measures: systolic, diastolic, and mean arterial pressure measured at both pre-and post-work exams for blood NanoString nCounter miRNA profiles. We used covariate-Adjusted linear mixedeffect models to examine associations between BP and increased miRNA expression in both pooled and risk factor-stratified analyses. Results Overall 43 miRNAs were associated with pre-work BP (FDR<0.05). In stratified analyses different but overlapping groups of miRNAs were associated with pre-work BP in truck drivershigh-BMI participants, and usual alcohol drinkers (FDR<0.05). Only four miRNAs were associated with post-work BP (FDR<0.05), in ever smokers. Conclusion Our results suggest that many miRNAs were significantly associated with BP in subgroups exposed to known hypertension risk factors. These findings shed light on the underlying molecular mechanisms of BP, and may assist with the development of a miRNA panel for early detection of hypertension.",
author = "Zhou Zhang and Joyce, {Brian Thomas} and Kresovich, {Jacob K.} and Yinan Zheng and Jia Zhong and Ruchi Patel and Wei Zhang and Lei Liu and Chang Dou and McCracken, {John P.} and Anaite{\^a} D{\~o}{\^A}az and Valeria Motta and Marco Sanchez-Guerra and Shurui Bian and Bertazzi, {Pier Alberto} and Joel Schwartz and Baccarelli, {Andrea A.} and Sheng Wang and Lifang Hou",
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Zhang, Z, Joyce, BT, Kresovich, JK, Zheng, Y, Zhong, J, Patel, R, Zhang, W, Liu, L, Dou, C, McCracken, JP, DõÂaz, A, Motta, V, Sanchez-Guerra, M, Bian, S, Bertazzi, PA, Schwartz, J, Baccarelli, AA, Wang, S & Hou, L 2017, 'Blood pressure and expression of microRNAs in whole blood', PloS one, vol. 12, no. 3, e0173550. https://doi.org/10.1371/journal.pone.0173550

Blood pressure and expression of microRNAs in whole blood. / Zhang, Zhou; Joyce, Brian Thomas; Kresovich, Jacob K.; Zheng, Yinan; Zhong, Jia; Patel, Ruchi; Zhang, Wei; Liu, Lei; Dou, Chang; McCracken, John P.; DõÂaz, Anaiteâ; Motta, Valeria; Sanchez-Guerra, Marco; Bian, Shurui; Bertazzi, Pier Alberto; Schwartz, Joel; Baccarelli, Andrea A.; Wang, Sheng; Hou, Lifang.

In: PloS one, Vol. 12, No. 3, e0173550, 01.03.2017.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Blood pressure and expression of microRNAs in whole blood

AU - Zhang, Zhou

AU - Joyce, Brian Thomas

AU - Kresovich, Jacob K.

AU - Zheng, Yinan

AU - Zhong, Jia

AU - Patel, Ruchi

AU - Zhang, Wei

AU - Liu, Lei

AU - Dou, Chang

AU - McCracken, John P.

AU - DõÂaz, Anaiteâ

AU - Motta, Valeria

AU - Sanchez-Guerra, Marco

AU - Bian, Shurui

AU - Bertazzi, Pier Alberto

AU - Schwartz, Joel

AU - Baccarelli, Andrea A.

AU - Wang, Sheng

AU - Hou, Lifang

PY - 2017/3/1

Y1 - 2017/3/1

N2 - Background Blood pressure (BP) is a complex, multifactorial clinical outcome driven by genetic susceptibility, behavioral choices, and environmental factors. Many molecular mechanisms have been proposed for the pathophysiology of high BP even as its prevalence continues to grow worldwide, increasing morbidity and marking it as a major public health concern. To address this, we evaluated miRNA profiling in blood leukocytes as potential biomarkers of BP and BP-related risk factors. Methods The Beijing Truck Driver Air Pollution Study included 60 truck drivers and 60 office workers examined in 2008. On two days separated by 1-2 weeks, we examined three BP measures: systolic, diastolic, and mean arterial pressure measured at both pre-and post-work exams for blood NanoString nCounter miRNA profiles. We used covariate-Adjusted linear mixedeffect models to examine associations between BP and increased miRNA expression in both pooled and risk factor-stratified analyses. Results Overall 43 miRNAs were associated with pre-work BP (FDR<0.05). In stratified analyses different but overlapping groups of miRNAs were associated with pre-work BP in truck drivershigh-BMI participants, and usual alcohol drinkers (FDR<0.05). Only four miRNAs were associated with post-work BP (FDR<0.05), in ever smokers. Conclusion Our results suggest that many miRNAs were significantly associated with BP in subgroups exposed to known hypertension risk factors. These findings shed light on the underlying molecular mechanisms of BP, and may assist with the development of a miRNA panel for early detection of hypertension.

AB - Background Blood pressure (BP) is a complex, multifactorial clinical outcome driven by genetic susceptibility, behavioral choices, and environmental factors. Many molecular mechanisms have been proposed for the pathophysiology of high BP even as its prevalence continues to grow worldwide, increasing morbidity and marking it as a major public health concern. To address this, we evaluated miRNA profiling in blood leukocytes as potential biomarkers of BP and BP-related risk factors. Methods The Beijing Truck Driver Air Pollution Study included 60 truck drivers and 60 office workers examined in 2008. On two days separated by 1-2 weeks, we examined three BP measures: systolic, diastolic, and mean arterial pressure measured at both pre-and post-work exams for blood NanoString nCounter miRNA profiles. We used covariate-Adjusted linear mixedeffect models to examine associations between BP and increased miRNA expression in both pooled and risk factor-stratified analyses. Results Overall 43 miRNAs were associated with pre-work BP (FDR<0.05). In stratified analyses different but overlapping groups of miRNAs were associated with pre-work BP in truck drivershigh-BMI participants, and usual alcohol drinkers (FDR<0.05). Only four miRNAs were associated with post-work BP (FDR<0.05), in ever smokers. Conclusion Our results suggest that many miRNAs were significantly associated with BP in subgroups exposed to known hypertension risk factors. These findings shed light on the underlying molecular mechanisms of BP, and may assist with the development of a miRNA panel for early detection of hypertension.

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Zhang Z, Joyce BT, Kresovich JK, Zheng Y, Zhong J, Patel R et al. Blood pressure and expression of microRNAs in whole blood. PloS one. 2017 Mar 1;12(3). e0173550. https://doi.org/10.1371/journal.pone.0173550