Bone morphogenic protein-2 gene therapy for mandibular distraction osteogenesis

Russell L. Ashinoff, Curtis L. Cetrulo, Robert D. Galiano, Michael Dobryansky, Kirit A. Bhatt, Daniel J. Ceradini, Joseph Michaels V, Joseph G. McCarthy, Geoffrey C. Gurtner*, George A. Csank

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

70 Scopus citations

Abstract

Distraction osteogenesis (DO) requires a long consolidation period and has a low but real failure rate. Bone morphogenic proteins (BMPs) accelerate bone deposition in fractures and critical-sized bone defects, but their effects on mandibular DO are unknown. We investigated the effect of local delivery of adenovirus containing the gene for BMP-2 (Adbmp-2) on mandibular DO in a rat model. Rats (n = 54) were distracted to 3 mm over 6 days. At the start of consolidation (POD 10), Adbmp-2 or adenovirus containing the lacZ gene (AdlacZ) was injected directly into the distraction zone. After 1, 2, and 4 weeks of consolidation, mandibles were evaluated for amount of bone deposition. Adbmp-2-treated specimens demonstrated an increased amount of new bone formation by radiographic, histologic, and histomorphometric analysis. This study demonstrates that local, adenovirally-mediated delivery of BMP-2 can increase bone deposition during DO, potentially shortening consolidation and enhancing DO in poorly healing mandibles, such as occurs postirradiation.

Original languageEnglish (US)
Pages (from-to)585-591
Number of pages7
JournalAnnals of plastic surgery
Volume52
Issue number6
DOIs
StatePublished - Jun 1 2004

Keywords

  • BMP-2
  • Distraction osteogenesis
  • Gene therapy

ASJC Scopus subject areas

  • Surgery

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