Bovine and equine peritubular and intertubular dentin

Stuart R Stock*, A. C. Deymier-Black, Arthur Veis, A. Telser, E. Lux, Z. Cai

*Corresponding author for this work

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Dentin contains 1-2 μm diameter tubules extending from the pulp cavity to near the junction with enamel. Peritubular dentin (PTD) borders the tubule lumens and is surrounded by intertubular dentin (ITD). Differences in PTD and ITD composition and microstructure remain poorly understood. Here, a (∼200 nm)2, 10.1 keV synchrotron X-ray beam maps X-ray fluorescence and X-ray diffraction simultaneously around tubules in 15-30 μm thick bovine and equine specimens. Increased Ca fluorescence surrounding tubule lumens confirms that PTD is present, and the relative intensities in PTD and ITD correspond to carbonated apatite (cAp) volume fraction of ∼0.8 in PTD vs. 0.65 assumed for ITD. In the PTD near the lumen edges, Zn intensity is strongly peaked, corresponding to a Zn content of ∼0.9 mg g-1 for an assumed concentration of ∼0.4 mg g-1 for ITD. In the equine specimen, the Zn K-edge position indicates that Zn2+ is present, similar to bovine dentin (Deymier-Black et al., 2013), and the above edge structure is consistent with spectra from macromolecules related to biomineralization. Transmission X-ray diffraction shows only cAp, and the 00.2 diffraction peak (Miller-Bravais indices) width is constant from ITD to the lumen edge. The cAp 00.2 average preferred orientation is axisymmetric (about the tubule axis) in both bovine and equine dentin, and the axisymmetric preferred orientation continues from ITD through the PTD to the tubule lumen. These data indicate that cAp structure does not vary from PTD to ITD.

Original languageEnglish (US)
Pages (from-to)3969-3977
Number of pages9
JournalActa Biomaterialia
Volume10
Issue number9
DOIs
StatePublished - Jan 1 2014

Fingerprint

Apatites
Apatite
Dentin
Horses
Fluorescence
Biomineralization
X ray diffraction
X rays
Enamels
Synchrotrons
Macromolecules
Pulp
Volume fraction
Diffraction
Microstructure
Chemical analysis
X-Ray Diffraction
X-Rays

Keywords

  • Dentin
  • Tubule
  • X-ray diffraction mapping
  • X-ray fluorescence mapping
  • Zinc

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering
  • Molecular Biology

Cite this

Stock, S. R., Deymier-Black, A. C., Veis, A., Telser, A., Lux, E., & Cai, Z. (2014). Bovine and equine peritubular and intertubular dentin. Acta Biomaterialia, 10(9), 3969-3977. https://doi.org/10.1016/j.actbio.2014.05.027
Stock, Stuart R ; Deymier-Black, A. C. ; Veis, Arthur ; Telser, A. ; Lux, E. ; Cai, Z. / Bovine and equine peritubular and intertubular dentin. In: Acta Biomaterialia. 2014 ; Vol. 10, No. 9. pp. 3969-3977.
@article{136c09fb6cb14aecb36b50ada0bc7a10,
title = "Bovine and equine peritubular and intertubular dentin",
abstract = "Dentin contains 1-2 μm diameter tubules extending from the pulp cavity to near the junction with enamel. Peritubular dentin (PTD) borders the tubule lumens and is surrounded by intertubular dentin (ITD). Differences in PTD and ITD composition and microstructure remain poorly understood. Here, a (∼200 nm)2, 10.1 keV synchrotron X-ray beam maps X-ray fluorescence and X-ray diffraction simultaneously around tubules in 15-30 μm thick bovine and equine specimens. Increased Ca fluorescence surrounding tubule lumens confirms that PTD is present, and the relative intensities in PTD and ITD correspond to carbonated apatite (cAp) volume fraction of ∼0.8 in PTD vs. 0.65 assumed for ITD. In the PTD near the lumen edges, Zn intensity is strongly peaked, corresponding to a Zn content of ∼0.9 mg g-1 for an assumed concentration of ∼0.4 mg g-1 for ITD. In the equine specimen, the Zn K-edge position indicates that Zn2+ is present, similar to bovine dentin (Deymier-Black et al., 2013), and the above edge structure is consistent with spectra from macromolecules related to biomineralization. Transmission X-ray diffraction shows only cAp, and the 00.2 diffraction peak (Miller-Bravais indices) width is constant from ITD to the lumen edge. The cAp 00.2 average preferred orientation is axisymmetric (about the tubule axis) in both bovine and equine dentin, and the axisymmetric preferred orientation continues from ITD through the PTD to the tubule lumen. These data indicate that cAp structure does not vary from PTD to ITD.",
keywords = "Dentin, Tubule, X-ray diffraction mapping, X-ray fluorescence mapping, Zinc",
author = "Stock, {Stuart R} and Deymier-Black, {A. C.} and Arthur Veis and A. Telser and E. Lux and Z. Cai",
year = "2014",
month = "1",
day = "1",
doi = "10.1016/j.actbio.2014.05.027",
language = "English (US)",
volume = "10",
pages = "3969--3977",
journal = "Acta Biomaterialia",
issn = "1742-7061",
publisher = "Elsevier BV",
number = "9",

}

Stock, SR, Deymier-Black, AC, Veis, A, Telser, A, Lux, E & Cai, Z 2014, 'Bovine and equine peritubular and intertubular dentin', Acta Biomaterialia, vol. 10, no. 9, pp. 3969-3977. https://doi.org/10.1016/j.actbio.2014.05.027

Bovine and equine peritubular and intertubular dentin. / Stock, Stuart R; Deymier-Black, A. C.; Veis, Arthur; Telser, A.; Lux, E.; Cai, Z.

In: Acta Biomaterialia, Vol. 10, No. 9, 01.01.2014, p. 3969-3977.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Bovine and equine peritubular and intertubular dentin

AU - Stock, Stuart R

AU - Deymier-Black, A. C.

AU - Veis, Arthur

AU - Telser, A.

AU - Lux, E.

AU - Cai, Z.

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Dentin contains 1-2 μm diameter tubules extending from the pulp cavity to near the junction with enamel. Peritubular dentin (PTD) borders the tubule lumens and is surrounded by intertubular dentin (ITD). Differences in PTD and ITD composition and microstructure remain poorly understood. Here, a (∼200 nm)2, 10.1 keV synchrotron X-ray beam maps X-ray fluorescence and X-ray diffraction simultaneously around tubules in 15-30 μm thick bovine and equine specimens. Increased Ca fluorescence surrounding tubule lumens confirms that PTD is present, and the relative intensities in PTD and ITD correspond to carbonated apatite (cAp) volume fraction of ∼0.8 in PTD vs. 0.65 assumed for ITD. In the PTD near the lumen edges, Zn intensity is strongly peaked, corresponding to a Zn content of ∼0.9 mg g-1 for an assumed concentration of ∼0.4 mg g-1 for ITD. In the equine specimen, the Zn K-edge position indicates that Zn2+ is present, similar to bovine dentin (Deymier-Black et al., 2013), and the above edge structure is consistent with spectra from macromolecules related to biomineralization. Transmission X-ray diffraction shows only cAp, and the 00.2 diffraction peak (Miller-Bravais indices) width is constant from ITD to the lumen edge. The cAp 00.2 average preferred orientation is axisymmetric (about the tubule axis) in both bovine and equine dentin, and the axisymmetric preferred orientation continues from ITD through the PTD to the tubule lumen. These data indicate that cAp structure does not vary from PTD to ITD.

AB - Dentin contains 1-2 μm diameter tubules extending from the pulp cavity to near the junction with enamel. Peritubular dentin (PTD) borders the tubule lumens and is surrounded by intertubular dentin (ITD). Differences in PTD and ITD composition and microstructure remain poorly understood. Here, a (∼200 nm)2, 10.1 keV synchrotron X-ray beam maps X-ray fluorescence and X-ray diffraction simultaneously around tubules in 15-30 μm thick bovine and equine specimens. Increased Ca fluorescence surrounding tubule lumens confirms that PTD is present, and the relative intensities in PTD and ITD correspond to carbonated apatite (cAp) volume fraction of ∼0.8 in PTD vs. 0.65 assumed for ITD. In the PTD near the lumen edges, Zn intensity is strongly peaked, corresponding to a Zn content of ∼0.9 mg g-1 for an assumed concentration of ∼0.4 mg g-1 for ITD. In the equine specimen, the Zn K-edge position indicates that Zn2+ is present, similar to bovine dentin (Deymier-Black et al., 2013), and the above edge structure is consistent with spectra from macromolecules related to biomineralization. Transmission X-ray diffraction shows only cAp, and the 00.2 diffraction peak (Miller-Bravais indices) width is constant from ITD to the lumen edge. The cAp 00.2 average preferred orientation is axisymmetric (about the tubule axis) in both bovine and equine dentin, and the axisymmetric preferred orientation continues from ITD through the PTD to the tubule lumen. These data indicate that cAp structure does not vary from PTD to ITD.

KW - Dentin

KW - Tubule

KW - X-ray diffraction mapping

KW - X-ray fluorescence mapping

KW - Zinc

UR - http://www.scopus.com/inward/record.url?scp=84905568550&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84905568550&partnerID=8YFLogxK

U2 - 10.1016/j.actbio.2014.05.027

DO - 10.1016/j.actbio.2014.05.027

M3 - Article

VL - 10

SP - 3969

EP - 3977

JO - Acta Biomaterialia

JF - Acta Biomaterialia

SN - 1742-7061

IS - 9

ER -

Stock SR, Deymier-Black AC, Veis A, Telser A, Lux E, Cai Z. Bovine and equine peritubular and intertubular dentin. Acta Biomaterialia. 2014 Jan 1;10(9):3969-3977. https://doi.org/10.1016/j.actbio.2014.05.027