BPH gene expression profile associated to prostate gland volume

Aurelien Descazeaud*, Mark A. Rubin, Matthias Hofer, Sunita Setlur, Nathalie Nikolaief, Francis Vacherot, Pascale Soyeux, Laurence Kheuang, Claude C. Abbou, Yves Allory, Alexandre De La Taille

*Corresponding author for this work

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

The aim of the current study was to analyze gene expression profiles in benign prostatic hyperplasia and to compare them with phenotypic properties. Thirty-seven specimens of benign prostatic hyperplasia were obtained from symptomatic patients undergoing surgery. RNA was extracted and hybridized to Affymetrix Chips containing 54,000 gene expression probes. Gene expression profiles were analyzed using cluster, TreeView, and significance analysis of microarrays softwares. In an initial unsupervised analysis, our 37 samples clustered hierarchically in 2 groups of 18 and 19 samples, respectively. Five clinical parameters were statistically different between the 2 groups: in group 1 compared with group 2, patients had larger prostate glands, had higher prostate specific antigen levels, were more likely to be treated by ot blockers, to be operated by prostatectomy, and to have major irritative symptoms. The sole independent parameter associated with this dichotome clustering, however, was the prostate gland volume. Therefore, the role of prostate volume was explored in a supervised analysis. Gene expression of prostate glands < 60 mL and > 60 mL were compared using significance analysis of microarrays and 227 genes were found differentially expressed between the 2 groups (> 2 change and false discovery rate of < 5%). Several specific pathways including growth factors genes, cell cycle genes, apoptose genes, inflammation genes, and androgen regulated genes, displayed major differences between small and large prostate glands.

Original languageEnglish (US)
Pages (from-to)207-213
Number of pages7
JournalDiagnostic Molecular Pathology
Volume17
Issue number4
DOIs
StatePublished - Dec 1 2008

Keywords

  • Benign prostatic hyperplasia
  • Gene expression
  • Prostate volume

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

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    Descazeaud, A., Rubin, M. A., Hofer, M., Setlur, S., Nikolaief, N., Vacherot, F., Soyeux, P., Kheuang, L., Abbou, C. C., Allory, Y., & De La Taille, A. (2008). BPH gene expression profile associated to prostate gland volume. Diagnostic Molecular Pathology, 17(4), 207-213. https://doi.org/10.1097/PDM.0b013e31816f6352