TY - JOUR
T1 - Bradykinin-induced phosphoinositide-dependent responses in protein kinase C down-regulated NCB-20 cells
AU - Okano, Yukio
AU - Higashida, Haruhiro
AU - Tao, Fu
AU - Sakai, Takayuki
AU - Nozawa, Yoshinori
PY - 1991
Y1 - 1991
N2 - Long-term (12 h) incubation of NCB-20 neuroblastoma × Chinese hamster brain cell hybrid cells with phorbol dibutylate caused a decrease in the enzyme activity of protein kinase C (PKC). Under these conditions, the formation of inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] and the mobilization of intracellular Ca2+ triggered by bradykinin (BK), were significantly increased. The alterations in BK-triggered membrane currents in the PKC down-regulated cells were also observed: enhancement of outward currents and decreased inward currents. These results strongly suggested that down-regulation of PKC enhanced BK-induced phosphoinositide metabolism by relieving from the negative feedback control operating between the receptor and phospholipase C, and that the increased Ca2+ responses due to accumulated Ins(1,4,5)P3 lead to enhanced outward currents.
AB - Long-term (12 h) incubation of NCB-20 neuroblastoma × Chinese hamster brain cell hybrid cells with phorbol dibutylate caused a decrease in the enzyme activity of protein kinase C (PKC). Under these conditions, the formation of inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] and the mobilization of intracellular Ca2+ triggered by bradykinin (BK), were significantly increased. The alterations in BK-triggered membrane currents in the PKC down-regulated cells were also observed: enhancement of outward currents and decreased inward currents. These results strongly suggested that down-regulation of PKC enhanced BK-induced phosphoinositide metabolism by relieving from the negative feedback control operating between the receptor and phospholipase C, and that the increased Ca2+ responses due to accumulated Ins(1,4,5)P3 lead to enhanced outward currents.
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U2 - 10.1016/0197-0186(91)90175-D
DO - 10.1016/0197-0186(91)90175-D
M3 - Article
AN - SCOPUS:0025977408
SN - 0197-0186
VL - 18
SP - 419
EP - 424
JO - Neurochemistry International
JF - Neurochemistry International
IS - 3
ER -