Bradykinin-induced phosphoinositide-dependent responses in protein kinase C down-regulated NCB-20 cells

Yukio Okano, Haruhiro Higashida, Fu Tao, Takayuki Sakai, Yoshinori Nozawa*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Long-term (12 h) incubation of NCB-20 neuroblastoma × Chinese hamster brain cell hybrid cells with phorbol dibutylate caused a decrease in the enzyme activity of protein kinase C (PKC). Under these conditions, the formation of inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] and the mobilization of intracellular Ca2+ triggered by bradykinin (BK), were significantly increased. The alterations in BK-triggered membrane currents in the PKC down-regulated cells were also observed: enhancement of outward currents and decreased inward currents. These results strongly suggested that down-regulation of PKC enhanced BK-induced phosphoinositide metabolism by relieving from the negative feedback control operating between the receptor and phospholipase C, and that the increased Ca2+ responses due to accumulated Ins(1,4,5)P3 lead to enhanced outward currents.

Original languageEnglish (US)
Pages (from-to)419-424
Number of pages6
JournalNeurochemistry International
Issue number3
StatePublished - 1991

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cell Biology

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