TY - JOUR
T1 - Bradykinin-induced translocation of protein kinases C in neuroblastoma NCB-20 cell
T2 - Dependence on 1,2-diacylglycerol content and free calcium
AU - Fu, Tao
AU - Okano, Yukio
AU - Hagiwara, Masatoshi
AU - Hidaka, Hiroyoshi
AU - Nozawa, Yoshinori
PY - 1989/8/15
Y1 - 1989/8/15
N2 - Bradykinin (BK)-induced production of 1,2-diacylglycerol (1,2-DG) and translocation of protein kinases C (PKCs) were examined in neuroblastoma-derived hybrid NCB-20 cells. Mass analysis of 1,2-DG exhibited a biphasic increase by 1 uM BK stimulation: the first transient phase and the second broad sustained phase. Among three subspecies of PKC expressed in these cells, types II and III were observed to translocate from cytosol to membrane in response to BK as well as PBt2 by Western blotting analysis. Type II translocated more rapidly and distinctly than type III. However, after treatment with quin 2/AM, the second phase of 1,2-DG formation completely disappeared and PKCs translocation by BK or PBt2 was completely abolished. BK-induced IP3(1,4,5) formation was temporally consistent with the first transient phase of 1,2-DG formation. These findings suggest that PKCs translocation by BK stimulation is caused by 1,2-DG produced not only via phosphoinositide metabolism, but via other phospholipid breakdown which is Ca2+-dependent.
AB - Bradykinin (BK)-induced production of 1,2-diacylglycerol (1,2-DG) and translocation of protein kinases C (PKCs) were examined in neuroblastoma-derived hybrid NCB-20 cells. Mass analysis of 1,2-DG exhibited a biphasic increase by 1 uM BK stimulation: the first transient phase and the second broad sustained phase. Among three subspecies of PKC expressed in these cells, types II and III were observed to translocate from cytosol to membrane in response to BK as well as PBt2 by Western blotting analysis. Type II translocated more rapidly and distinctly than type III. However, after treatment with quin 2/AM, the second phase of 1,2-DG formation completely disappeared and PKCs translocation by BK or PBt2 was completely abolished. BK-induced IP3(1,4,5) formation was temporally consistent with the first transient phase of 1,2-DG formation. These findings suggest that PKCs translocation by BK stimulation is caused by 1,2-DG produced not only via phosphoinositide metabolism, but via other phospholipid breakdown which is Ca2+-dependent.
UR - http://www.scopus.com/inward/record.url?scp=0024325187&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024325187&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(89)90812-7
DO - 10.1016/0006-291X(89)90812-7
M3 - Article
C2 - 2764933
AN - SCOPUS:0024325187
SN - 0006-291X
VL - 162
SP - 1279
EP - 1286
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -