External application of bradykinin (BK) to mouse neuroblastoma × mouse fibroblast hybrid NL308 cells and mouse neuroblastoma × rat glioma hybrid NG108–15 cells produced a transient outward (hyperpolarizing) current. In NG108–15 cells, BK also induced an inward (depolarizing) current associated with a decrease in input membrane conductance, which results from the inhibition of a voltage‐sensitive potassium current, the M‐current. However, in NL308 cells, either no depolarization was elicited by BK or, even if the BK‐induced depolarization was evoked, it was associated with an increased conductance. To explain the above difference, the intracellular second messenger system of NL308 cells was examined in detail. BK induced the rapid accumulation (three‐ to fivefold higher than the control level) of inositol 1,4,5‐trisphosphate (InsP3) in NL308 cells. The cytosolic Ca2+ concentration was also elevated to 540 nM from 180 nM at a basal level. This seems to be enough to activate a voltage‐independent and Ca2+‐sensitive K+ current, resulting in the hyperpolarization. Intracellular injection of InsP3 replicated the hyperpolarization. NL308 cells possess protein kinase C (C‐kinase), with specific activities of C‐kinase in cytosolic and membrane fractions being 233 and 24 pmol/min/mg protein, respectively. The activity associated with particulates became higher after phorbol dibutyrate (PDBu) treatment. But NL308 cells did not show the characteristic inward relaxation by step hyperpolarizations and the outward rectification in the current‐voltage relationship, indicating that the M current is deficient in NL308 cells. Therefore, application of PDBu failed to mimic the inward current. The results suggest the role of InsP3 and C‐kinase in controlling two K+ currents.
- Ca‐dependent K currents
- Neuroblastoma × fibroblast hybrid cells
- PI turnover
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience