Bre1, an E3 ubiquitin ligase required for recruitment and substrate selection of Rad6 at a promoter

Adam Wood; Nevan J. Krogan; Jim Dover; Jessica Schneider; Jonathan Heidt; Marry Ann Boateng; Kimberly Dean; Ashkan Golshani; Yi Zhang; Jack F. Greenblatt; Mark Johnston; Ali Shilatifard

doi:10.1016/S1097-2765(02)00802-X

Bre1, an E3 ubiquitin ligase required for recruitment and substrate selection of Rad6 at a promoter

Adam Wood, Nevan J. Krogan, Jim Dover, Jessica Schneider, Jonathan Heidt, Marry Ann Boateng, Kimberly Dean, Ashkan Golshani, Yi Zhang, Jack F. Greenblatt, Mark Johnston, Ali Shilatifard^*

^*Corresponding author for this work

Biochemistry and Molecular Genetics

Research output: Contribution to journal › Article › peer-review

438 Scopus citations

Abstract

Ubiquitination of histone H2B catalyzed by Rad6 is required for methylation of histone H3 by COMPASS. We identified Bre1 as the probable E3 for Rad6's role in transcription. Bre1 contains a C3HC4 (RING) finger and is present with Rad6 in a complex. The RING finger of Bre1 is required for ubiquitination of histone H2B, methylation of lysine 4 and 79 of H3 and for telomeric silencing. Chromatin immunoprecipitation experiments indicated that both Rad6 and Bre1 are recruited to a promoter. Bre1 is essential for this recruitment of Rad6 and is dedicated to the transcriptional pathway of Rad6. These results suggest that Bre1 is the likely E3 enzyme that directs Rad6 to modify chromatin and ultimately to affect gene expression.

Original language	English (US)
Pages (from-to)	267-274
Number of pages	8
Journal	Molecular cell
Volume	11
Issue number	1
DOIs	https://doi.org/10.1016/S1097-2765(02)00802-X
State	Published - Jan 1 2003

Funding

We thank Drs. Kevin Struhl and Huck Ng for their generous gift of FLAG-tagged H2B. The authors also thank Drs. Chris Brower, Alexander Varshavsky, and Joan and Ronald Conway for helpful advice and discussions. This work was supported in part by grants from the American Cancer Society (RP69921801) and the National Institutes of Health (1R01CA089455), and by a Mallinckrodt Foundation Award to A.S.'s laboratory. Work in M.J.'s laboratory was supported by the James S. McDonnell Foundation. A.S. is a Scholar of the Leukemia & Lymphoma Society.

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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10.1016/S1097-2765(02)00802-X

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title = "Bre1, an E3 ubiquitin ligase required for recruitment and substrate selection of Rad6 at a promoter",

abstract = "Ubiquitination of histone H2B catalyzed by Rad6 is required for methylation of histone H3 by COMPASS. We identified Bre1 as the probable E3 for Rad6's role in transcription. Bre1 contains a C3HC4 (RING) finger and is present with Rad6 in a complex. The RING finger of Bre1 is required for ubiquitination of histone H2B, methylation of lysine 4 and 79 of H3 and for telomeric silencing. Chromatin immunoprecipitation experiments indicated that both Rad6 and Bre1 are recruited to a promoter. Bre1 is essential for this recruitment of Rad6 and is dedicated to the transcriptional pathway of Rad6. These results suggest that Bre1 is the likely E3 enzyme that directs Rad6 to modify chromatin and ultimately to affect gene expression.",

author = "Adam Wood and Krogan, {Nevan J.} and Jim Dover and Jessica Schneider and Jonathan Heidt and Boateng, {Marry Ann} and Kimberly Dean and Ashkan Golshani and Yi Zhang and Greenblatt, {Jack F.} and Mark Johnston and Ali Shilatifard",

note = "Funding Information: We thank Drs. Kevin Struhl and Huck Ng for their generous gift of FLAG-tagged H2B. The authors also thank Drs. Chris Brower, Alexander Varshavsky, and Joan and Ronald Conway for helpful advice and discussions. This work was supported in part by grants from the American Cancer Society (RP69921801) and the National Institutes of Health (1R01CA089455), and by a Mallinckrodt Foundation Award to A.S.'s laboratory. Work in M.J.'s laboratory was supported by the James S. McDonnell Foundation. A.S. is a Scholar of the Leukemia & Lymphoma Society.",

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AU - Wood, Adam

AU - Krogan, Nevan J.

AU - Dover, Jim

AU - Schneider, Jessica

AU - Heidt, Jonathan

AU - Boateng, Marry Ann

AU - Dean, Kimberly

AU - Golshani, Ashkan

AU - Zhang, Yi

AU - Greenblatt, Jack F.

AU - Johnston, Mark

AU - Shilatifard, Ali

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N2 - Ubiquitination of histone H2B catalyzed by Rad6 is required for methylation of histone H3 by COMPASS. We identified Bre1 as the probable E3 for Rad6's role in transcription. Bre1 contains a C3HC4 (RING) finger and is present with Rad6 in a complex. The RING finger of Bre1 is required for ubiquitination of histone H2B, methylation of lysine 4 and 79 of H3 and for telomeric silencing. Chromatin immunoprecipitation experiments indicated that both Rad6 and Bre1 are recruited to a promoter. Bre1 is essential for this recruitment of Rad6 and is dedicated to the transcriptional pathway of Rad6. These results suggest that Bre1 is the likely E3 enzyme that directs Rad6 to modify chromatin and ultimately to affect gene expression.

AB - Ubiquitination of histone H2B catalyzed by Rad6 is required for methylation of histone H3 by COMPASS. We identified Bre1 as the probable E3 for Rad6's role in transcription. Bre1 contains a C3HC4 (RING) finger and is present with Rad6 in a complex. The RING finger of Bre1 is required for ubiquitination of histone H2B, methylation of lysine 4 and 79 of H3 and for telomeric silencing. Chromatin immunoprecipitation experiments indicated that both Rad6 and Bre1 are recruited to a promoter. Bre1 is essential for this recruitment of Rad6 and is dedicated to the transcriptional pathway of Rad6. These results suggest that Bre1 is the likely E3 enzyme that directs Rad6 to modify chromatin and ultimately to affect gene expression.

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Bre1, an E3 ubiquitin ligase required for recruitment and substrate selection of Rad6 at a promoter

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