Breast cancer stem cell isolation

Xuanmao Jiao, Albert A. Rizvanov, Massimo Cristofanilli, Regina R. Miftakhova, Richard G. Pestell

Research output: Chapter in Book/Report/Conference proceedingChapter

25 Scopus citations


Cells within the tumor are highly heterogeneous. Only a small portion of the cells within the tumor is capable to generate a new tumor. These cells are called cancer stem cells. Theoretically, cancer stem cells are originally from normal stem cells or early progenitor cells which accumulate the random mutations and undergo an altered version of the normal differentiation process. The cancer stem cell drives tumor progression and its recurrence. Thus, the technique to identify and purify the cancer stem cell is the key in any cancer stem cell research. In this protocol, we provide the basic technology of identification and purification of breast cancer stem cells as well as further functional assays to help the researchers achieve their research goals.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages15
StatePublished - Jan 1 2016

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Breast cancer stem cells
  • Circulating tumor cells
  • Fluorescenceactivated cell sorting (FACS)
  • Mammosphere formation
  • Transplantation assays

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology


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