Abstract
Amphiphilic macromolecules with multiple borondipyrromethene (BODIPY) chromophores appended to a common poly(methacrylate) backbone were synthesized by the random co-polymerization of appropriate methacrylate monomers. The resulting polymers incorporate also hydrophilic oligo(ethylene glycol) and hydrophobic decyl side chains designed to impose aqueous solubility and insulate the chromophoric components from each other respectively. The presence of multiple chromophores translates into a significant enhancement in molar absorption coefficient, relative to a model BODIPY monomer. The effective insulation of the fluorophores minimizes interchromophoric interactions and mitigates depressive effects on the fluorescence quantum yield. The overall result is a 6-fold enhancement in brightness, relative to the model monomer. These macromolecular probes can be injected into live Caenorhabditis elegans to allow their visualization with a 4-fold increase in signal intensity, relative to the model system. Furthermore, they can be conjugated to secondary antibodies, under standard amide-coupling conditions, with negligible influence on the binding affinity of the biomoleucles to allow the implementation of immunolabeling protocols.
Original language | English (US) |
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Title of host publication | Colloidal Nanoparticles for Biomedical Applications XIII |
Editors | Wolfgang J. Parak, Marek Osinski, Xing-Jie Liang |
Publisher | SPIE |
ISBN (Electronic) | 9781510614994 |
DOIs | |
State | Published - 2018 |
Event | Colloidal Nanoparticles for Biomedical Applications XIII 2018 - San Francisco, United States Duration: Jan 27 2018 → Jan 29 2018 |
Publication series
Name | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
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Volume | 10507 |
ISSN (Print) | 1605-7422 |
Conference
Conference | Colloidal Nanoparticles for Biomedical Applications XIII 2018 |
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Country/Territory | United States |
City | San Francisco |
Period | 1/27/18 → 1/29/18 |
Funding
The National Science Foundation (CHE-1505885) and the National Institutes of Health (NS086932) are acknowledged for financial support.
Keywords
- Amphiphilic polymers
- BODIPYs
- fluorescence imaging
- immunolabeling
- macromolecular probes
- nanoparticles
- self-assembly
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
- Radiology Nuclear Medicine and imaging
- Biomaterials