Butyrylcholinesterase is associated with β-amyloid plaques in the transgenic APPSWE/PSEN1dE9 mouse model of alzheimer disease

Sultan Darvesh*, Meghan K. Cash, George Andrew Reid, Earl Martin, Arnold Mitnitski, Changiz Geula

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

117 Scopus citations


Histochemical analysis of Alzheimer disease (AD) brain tissues indicates that butyrylcholinesterase (BuChE) is present in β-amyloid (Aβ) plaques. The role of BuChE in AD pathology is unknown, but an animal model developing similar BuChE-associated Aβ plaques could provide insights. The APPSWE/PSEN1dE9 transgenic mouse (ADTg), which develops Aβ plaques, was examined to determine if BuChE associates with these plaques, as in AD. We found that in mature ADTg mice, BuChE activity associated with Aβ plaques. The Aβ-, thioflavin-S- and BuChE-positive plaques mainly accumulated in the olfactory structures, cerebral cortex, hippocampal formation, amygdala, and cerebellum. No plaques were stained for acetylcholinesterase activity. The distribution and abundance of plaque staining in ADTg closely resembled many aspects of plaque staining in AD. Butyrylcholinesterase staining consistently showed fewer plaques than were detected with Aβ immunostaining but a greater number of plaques than were visualized with thioflavin-S. Double-labeling experiments demonstrated that all BuChE-positive plaques were Aβ positive, whereas only some BuChE-positive plaques were thioflavin-S positive. These observations suggest that BuChE is associated with a subpopulation of Aβ plaques and may play a role in AD plaque maturation. A further study of this animal model could clarify the role of BuChE in AD pathology.

Original languageEnglish (US)
Pages (from-to)2-14
Number of pages13
JournalJournal of neuropathology and experimental neurology
Issue number1
StatePublished - Jan 2012


  • Alzheimer disease
  • Amygdala
  • Cerebellum
  • Cerebral cortex
  • Cholinesterases
  • Hippocampus
  • Olfactory structures
  • Thioflavin-S
  • β-amyloid

ASJC Scopus subject areas

  • Clinical Neurology
  • Neurology
  • Cellular and Molecular Neuroscience
  • Pathology and Forensic Medicine


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