C5aR-mediated myocardial ischemia/reperfusion injury

Haimou Zhang, Gangjian Qin, Gang Liang, Jinan Li, Robert A. Barrington, Dong xu Liu*

*Corresponding author for this work

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

The complement system activation can mediate myocardial ischemia and reperfusion (I/R). Inhibition of C5a activity reveals attenuation of I/R-induced myocardial infarct size. However, the contribution of C5a receptor (C5aR) to I/R injury remains to be unknown. Here, we reported that both mRNA and protein for the C5aR were constitutively expressed on cardiomyocytes and upregulated as a function of time after I/R-induced myocardial cell injury in mice. Blockade of C5aR markedly decreased microvascular permeability in ischemic myocardial area and leukocyte adherence to coronary artery endothelium. Importantly, the blocking of C5aR with an anti-C5aR antibody was associated with inhibition in activation of protein kinase C δ (PKC-δ) and induction of PKC-mediated mitogen-activated protein kinase phosphatases-1 (MKP-1) leading to the increased activity of p42/p44 mitogen-activated protein (MAP) kinase cascade. These data provide evidence that C5aR-mediated myocardial cell injury is an important pathogenic factor, and that C5aR blockade may be useful therapeutic targets for the prevention of myocardial I/R injury.

Original languageEnglish (US)
Pages (from-to)446-452
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume357
Issue number2
DOIs
StatePublished - Jun 1 2007

Keywords

  • C5aR
  • Complement
  • Ischemia/reperfusion
  • Myocardial cell
  • Signaling

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Zhang, H., Qin, G., Liang, G., Li, J., Barrington, R. A., & Liu, D. X. (2007). C5aR-mediated myocardial ischemia/reperfusion injury. Biochemical and Biophysical Research Communications, 357(2), 446-452. https://doi.org/10.1016/j.bbrc.2007.03.152