TY - JOUR
T1 - Cadmium-induced leydig cell tumors of rat testis
T2 - Morphologic and cytochemical study
AU - Reddy, Janardan
AU - Svoboda, Donald
AU - Azarnoff, Daniel
AU - Dawar, Ramesh
N1 - Funding Information:
1 Received March 5, 1973; accepted May 14, 1973. 2 Supported in part by Public Health Service grants GMI5956 from the National Institute of General Medical Sciences, and CA5680 from the National Cancer Institute. 3 Department of Pathology and Oncology and Clinical Pharmacology-Toxicology Center, Departments of Medicine and Pharmacology, University of Kansas Medical Center, Kansas City, Mo. 66103. 4 We thank Mr. J. D. Prasad, Mr. C. Sittler, Ms. Dianne Knox, and Ms. Faye Brady for their technical assistance, and Mrs. Ella M. Olson for typing the manuscript.
PY - 1973/9
Y1 - 1973/9
N2 - Leydig cell tumors of the rat testis induced by a single subcutaneous injection of cadmium chloride were studied by electron microscope and histochemical methods. The tumor cells contained abundant agranular endoplasmic reticulum appearing as a network of interconnecting tubules of uniform diameter. The mitochondria were numerous, varied in size, and contained compact vesicular inner structure. In some tumor cells the mitochondrial cristae were not elaborate. The cytoplasmic lipid appeared as numerous vacuoles and was more prominent in larger tumors. Many microbodies (peroxisomes) were also observed in tumor cells. Some possessed tubular or cylindrical inclusions identical to those in peroxisomes of kidney and spontaneous Leydig cell tumors of the rat testis. Several microbodies also revealed the presence of an amorphous, highly electron dense center. 3/3-Hydroxy steroid dehydrogenase activity was localized in these tumors histochemically. These observations indicate that cadmium-induced Leydig cell tumors in the rat testis are capable of steroidogenesis.—J Natl Cancer Inst 51: 891-903, 1973.
AB - Leydig cell tumors of the rat testis induced by a single subcutaneous injection of cadmium chloride were studied by electron microscope and histochemical methods. The tumor cells contained abundant agranular endoplasmic reticulum appearing as a network of interconnecting tubules of uniform diameter. The mitochondria were numerous, varied in size, and contained compact vesicular inner structure. In some tumor cells the mitochondrial cristae were not elaborate. The cytoplasmic lipid appeared as numerous vacuoles and was more prominent in larger tumors. Many microbodies (peroxisomes) were also observed in tumor cells. Some possessed tubular or cylindrical inclusions identical to those in peroxisomes of kidney and spontaneous Leydig cell tumors of the rat testis. Several microbodies also revealed the presence of an amorphous, highly electron dense center. 3/3-Hydroxy steroid dehydrogenase activity was localized in these tumors histochemically. These observations indicate that cadmium-induced Leydig cell tumors in the rat testis are capable of steroidogenesis.—J Natl Cancer Inst 51: 891-903, 1973.
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U2 - 10.1093/jnci/51.3.891
DO - 10.1093/jnci/51.3.891
M3 - Article
C2 - 4743560
AN - SCOPUS:0015860833
SN - 0027-8874
VL - 51
SP - 891
EP - 903
JO - Journal of the National Cancer Institute
JF - Journal of the National Cancer Institute
IS - 3
ER -
