TY - JOUR
T1 - Calbindin-D28K, parvalbumin, and calretinin in young and aged human locus coeruleus
AU - Lamerand, Sydney
AU - Shahidehpour, Ryan
AU - Ayala, Ivan
AU - Gefen, Tamar
AU - Mesulam, M. Marsel
AU - Bigio, Eileen
AU - Geula, Changiz
N1 - Funding Information:
This work was supported in part by a Zenith Fellows Award (C.G.) from the Alzheimer's Association; grants from the National Institute on Aging ( AG014706 and AG027141 ), National Alzheimer's Coordinating Center (NACC; U01 AG016976 ), and by an Alzheimer's Disease Center grant ( AG013854 ).
Publisher Copyright:
© 2020 Elsevier Inc.
PY - 2020/10
Y1 - 2020/10
N2 - Certain neuronal populations, including basal forebrain cholinergic neurons (BFCN) and noradrenergic neurons of the locus coeruleus (LC), are selectively vulnerable to pathology and loss early in the course of aging and Alzheimer's disease (AD). Human BFCN show substantial loss of the calcium-binding protein (CBP), calbindin-D28K (CB), during normal aging, which is associated with formation of neurofibrillary tangles and BFCN loss in AD. Here we determined if, similar to the BFCN, LC neurons contain CB or the other 2 ubiquitous CBPs parvalbumin and calretinin, and whether these proteins display an age-related loss from LC neurons. Immunostaining for CBP and tyrosine hydroxylase, a marker of catecholaminergic neurons, was used in sections from the LC of young and aged human brains. Parvalbumin and calretinin immunoreactivities were completely absent from human LC neurons. A subpopulation of LC neurons (~10%) contained CB immunoreactivity. Quantitative analysis revealed no age-related loss of CB from LC neurons. Thus, unlike the BFCN, age-related loss of CB does not figure prominently in the selective vulnerability of LC neurons to degeneration in AD.
AB - Certain neuronal populations, including basal forebrain cholinergic neurons (BFCN) and noradrenergic neurons of the locus coeruleus (LC), are selectively vulnerable to pathology and loss early in the course of aging and Alzheimer's disease (AD). Human BFCN show substantial loss of the calcium-binding protein (CBP), calbindin-D28K (CB), during normal aging, which is associated with formation of neurofibrillary tangles and BFCN loss in AD. Here we determined if, similar to the BFCN, LC neurons contain CB or the other 2 ubiquitous CBPs parvalbumin and calretinin, and whether these proteins display an age-related loss from LC neurons. Immunostaining for CBP and tyrosine hydroxylase, a marker of catecholaminergic neurons, was used in sections from the LC of young and aged human brains. Parvalbumin and calretinin immunoreactivities were completely absent from human LC neurons. A subpopulation of LC neurons (~10%) contained CB immunoreactivity. Quantitative analysis revealed no age-related loss of CB from LC neurons. Thus, unlike the BFCN, age-related loss of CB does not figure prominently in the selective vulnerability of LC neurons to degeneration in AD.
KW - Alzheimer's disease
KW - Calbindin-D
KW - Calcium-binding proteins
KW - Locus coeruleus
KW - Noradrenergic
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U2 - 10.1016/j.neurobiolaging.2020.06.006
DO - 10.1016/j.neurobiolaging.2020.06.006
M3 - Article
C2 - 32663717
AN - SCOPUS:85087686781
SN - 0197-4580
VL - 94
SP - 243
EP - 249
JO - Neurobiology of Aging
JF - Neurobiology of Aging
ER -