TY - JOUR
T1 - Calcium-activated potassium channels in chondrocytes
AU - Grandolfo, M.
AU - D'Andrea, P.
AU - Martina, M.
AU - Ruzzier, F.
AU - Vittur, F.
N1 - Funding Information:
This work was supported by research grants from CNR and MURST, Italy. We thank Miss M.T. Nicodemo for her valid technical assistance. We also acknowledge the Fondo per lo Studio delle Malattie de1 Fegato for use of the spectrofluorometer, and the Uanetto Delicatessen Factory for the generous supply of pip scapulas.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1992/2/14
Y1 - 1992/2/14
N2 - The presence of calcium-activated potassium channels in chondrocytes of growing cartilage was tested. Results obtained with fura-2 on cultured resting chondrocytes indicate that the cells respond to an elevation of extracellular calcium concentration ( [Ca2+]o ) from 0.1 to 2 mM increasing the intracellular concentration of the ion ( [Ca2+]i ) from 117 to 187 nM. This increment may be blocked by 3μM La3+. Patch clamp experiments in cell-attached configuration showed that, when [Ca2+]i rises, the open probability (Po) of the K+ channels increases. Increments in both Po and unitary currents of the K+ channels can be obtained after applying 2.5μM A23187 with 2mM [Ca2+]o. Hence, the results demonstrate that, in chondrocytes, a class of Ca2+-activated K+ channels is present and their activity is related to an increase of [Ca2+]i.
AB - The presence of calcium-activated potassium channels in chondrocytes of growing cartilage was tested. Results obtained with fura-2 on cultured resting chondrocytes indicate that the cells respond to an elevation of extracellular calcium concentration ( [Ca2+]o ) from 0.1 to 2 mM increasing the intracellular concentration of the ion ( [Ca2+]i ) from 117 to 187 nM. This increment may be blocked by 3μM La3+. Patch clamp experiments in cell-attached configuration showed that, when [Ca2+]i rises, the open probability (Po) of the K+ channels increases. Increments in both Po and unitary currents of the K+ channels can be obtained after applying 2.5μM A23187 with 2mM [Ca2+]o. Hence, the results demonstrate that, in chondrocytes, a class of Ca2+-activated K+ channels is present and their activity is related to an increase of [Ca2+]i.
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U2 - 10.1016/0006-291X(92)91893-U
DO - 10.1016/0006-291X(92)91893-U
M3 - Article
C2 - 1540186
AN - SCOPUS:0026523723
SN - 0006-291X
VL - 182
SP - 1429
EP - 1434
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -