TY - JOUR
T1 - Calcium-activated potassium channels in chondrocytes
AU - Grandolfo, M.
AU - D'Andrea, P.
AU - Martina, M.
AU - Ruzzier, F.
AU - Vittur, F.
PY - 1992/2/14
Y1 - 1992/2/14
N2 - The presence of calcium-activated potassium channels in chondrocytes of growing cartilage was tested. Results obtained with fura-2 on cultured resting chondrocytes indicate that the cells respond to an elevation of extracellular calcium concentration ( [Ca2+]o ) from 0.1 to 2 mM increasing the intracellular concentration of the ion ( [Ca2+]i ) from 117 to 187 nM. This increment may be blocked by 3μM La3+. Patch clamp experiments in cell-attached configuration showed that, when [Ca2+]i rises, the open probability (Po) of the K+ channels increases. Increments in both Po and unitary currents of the K+ channels can be obtained after applying 2.5μM A23187 with 2mM [Ca2+]o. Hence, the results demonstrate that, in chondrocytes, a class of Ca2+-activated K+ channels is present and their activity is related to an increase of [Ca2+]i.
AB - The presence of calcium-activated potassium channels in chondrocytes of growing cartilage was tested. Results obtained with fura-2 on cultured resting chondrocytes indicate that the cells respond to an elevation of extracellular calcium concentration ( [Ca2+]o ) from 0.1 to 2 mM increasing the intracellular concentration of the ion ( [Ca2+]i ) from 117 to 187 nM. This increment may be blocked by 3μM La3+. Patch clamp experiments in cell-attached configuration showed that, when [Ca2+]i rises, the open probability (Po) of the K+ channels increases. Increments in both Po and unitary currents of the K+ channels can be obtained after applying 2.5μM A23187 with 2mM [Ca2+]o. Hence, the results demonstrate that, in chondrocytes, a class of Ca2+-activated K+ channels is present and their activity is related to an increase of [Ca2+]i.
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U2 - 10.1016/0006-291X(92)91893-U
DO - 10.1016/0006-291X(92)91893-U
M3 - Article
C2 - 1540186
AN - SCOPUS:0026523723
VL - 182
SP - 1429
EP - 1434
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -