Calcium dependence of glutamate receptor-evoked alkaline shifts in hippocampus

Sabrina E. Smith, Jay A. Gottfried, Joseph C T Chen, Mitchell Chesler*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

GLUTAMATE receptor activation induces an extracellular alkalinization in rodent hippocampus. We studied its Ca2+ dependence and pharmacology in hippocampal slices. Glutamate-evoked alkaline shifts were blocked by 0 Ca2+ saline. Alkalinizations induced by AMPA (α-amino-3-hydroxy-5-methylisoxazole-4-propionic acid) and NMDA (N-methyl-D-aspartate) were abolished by 20 μM CNQX (6-cyano-7-nitro-nitroquinoxaline-2,3-dione) and 50 μM APV (DL-2-amino-5-phosphonovaIerate), respectively. The AMPA-and NMDA-evoked alkaline shifts were blocked by 0 Ca2+, however, AMPA-induced [K+]o elevation was unaffected. These data suggest that the glutamate receptor-channel does not mediate H+ influx, and support a role for Ca2+-H+ exchange.

Original languageEnglish (US)
Pages (from-to)2441-2445
Number of pages5
JournalNeuroreport
Volume5
Issue number18
DOIs
StatePublished - Dec 1994

Keywords

  • Ampa
  • Ca-H
  • Excitatory synaptic transmission
  • Extracellular pH
  • Glutamate
  • Glutamate uptake
  • Hippocampus
  • Nmda

ASJC Scopus subject areas

  • Neuroscience(all)

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