Calcium dependent regulatory protein of cyclic nucleotide metabolism in normal and transformed chicken embryo fibroblasts

D. M. Watterson, L. J. Van Eldik, R. E. Smith, T. C. Vanaman

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Abstract

The concentration of a calcium binding protein modulator of 3':5' cyclic nucleotide phosphodiesterase (3':5' cyclic nucleotide 5' nucleotidohydrolase) activity is increased in chicken embryo fibroblasts upon transformation by Rous sarcoma virus. This modulator protein from fibroblasts, which has roughly the same molecular size, charge, and functional properties as that isolated from chicken brain, comprises approximately 1.32% of the soluble protein in homogenates of fibroblasts infected and transformed by Rous sarcoma virus. In comparison, the modulator comprises approximately 0.30% of the soluble protein in homogenates of normal fibroblasts from confluent cultures and 0.36% of the soluble protein in homogenates of fibroblasts infected with a transformation defective mutant of Rous sarcoma virus. Modulator levels in normal fibroblasts at subconfluent cell densities are 0.42-0.76% of the homogenate soluble protein, i.e., between that found in confluent normal fibroblasts and in fibroblasts transformed by Rous sarcoma virus. These observations suggest that the levels of the modulator protein are elevated under conditions in which chicken embryo fibroblasts are undergoing rapid growth and have decreased adenosine 3':5' cyclic monophosphate levels.

Original languageEnglish (US)
Pages (from-to)2711-2715
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume73
Issue number8
DOIs
StatePublished - 1976

ASJC Scopus subject areas

  • General

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