Calcium entry induces mitochondrial oxidant stress in vagal neurons at risk in Parkinson's disease

Joshua A. Goldberg, Jaime N. Guzman, Chad M. Estep, Ema Ilijic, Jyothisri Kondapalli, Javier Sanchez-Padilla, D. James Surmeier*

*Corresponding author for this work

Research output: Contribution to journalArticle

95 Scopus citations

Abstract

Mitochondrial oxidant stress is widely viewed as being critical to pathogenesis in Parkinson's disease. But the origins of this stress are poorly defined. One possibility is that it arises from the metabolic demands associated with regenerative activity. To test this hypothesis, we characterized neurons in the dorsal motor nucleus of the vagus (DMV), a population of cholinergic neurons that show signs of pathology in the early stages of Parkinson's disease, in mouse brain slices. DMV neurons were slow, autonomous pacemakers with broad spikes, leading to calcium entry that was weakly buffered. Using a transgenic mouse expressing a redox-sensitive optical probe targeted to the mitochondrial matrix, we found that calcium entry during pacemaking created a basal mitochondrial oxidant stress. Knocking out DJ-1 (also known as PARK7), a gene associated with early-onset Parkinson's disease, exacerbated this stress. These results point to a common mechanism underlying mitochondrial oxidant stress in Parkinson's disease and a therapeutic strategy to ameliorate it.

Original languageEnglish (US)
Pages (from-to)1414-1421
Number of pages8
JournalNature Neuroscience
Volume15
Issue number10
DOIs
StatePublished - Oct 1 2012

ASJC Scopus subject areas

  • Neuroscience(all)

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