Calcium oscillation associated with reduced protein kinase C activities in ras-transformed NIH3T3 cells

Tao Fu; Yoshikazu Sugimoto; Tohru Oki; Shigeko Murakami; Yukio Okano; Yoshinori Nozawa

doi:10.1016/0014-5793(91)80407-T

Calcium oscillation associated with reduced protein kinase C activities in ras-transformed NIH3T3 cells

Tao Fu, Yoshikazu Sugimoto, Tohru Oki, Shigeko Murakami, Yukio Okano, Yoshinori Nozawa^*

^*Corresponding author for this work

Pathology

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

We show here novel intracellular Ca²⁺ oscillation in ν-K-ras-transformed NIH3T3 cells induced by mitogenic peptide hormones, bradykinin and bombesin, as well as fetal calf serum. Induction of the Ca²⁺ oscillation is strongly correlated with the malignant properties and inversely with PKC activities in vitro and in vivo. These results suggest that the mitogen-induced Ca²⁺ oscillation is negatively regulated by PKC, which modulates Ca²⁺ influx in ν-K-ras-transformed NIH3T3 cells.

Original language	English (US)
Pages (from-to)	263-266
Number of pages	4
Journal	FEBS Letters
Volume	281
Issue number	1-2
DOIs	https://doi.org/10.1016/0014-5793(91)80407-T
State	Published - Apr 9 1991

Keywords

Ca oscillation
Protein kinase C
ras-Transformed fibroblast

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(91)80407-T

Cite this

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title = "Calcium oscillation associated with reduced protein kinase C activities in ras-transformed NIH3T3 cells",

abstract = "We show here novel intracellular Ca2+ oscillation in ν-K-ras-transformed NIH3T3 cells induced by mitogenic peptide hormones, bradykinin and bombesin, as well as fetal calf serum. Induction of the Ca2+ oscillation is strongly correlated with the malignant properties and inversely with PKC activities in vitro and in vivo. These results suggest that the mitogen-induced Ca2+ oscillation is negatively regulated by PKC, which modulates Ca2+ influx in ν-K-ras-transformed NIH3T3 cells.",

keywords = "Ca oscillation, Protein kinase C, ras-Transformed fibroblast",

author = "Tao Fu and Yoshikazu Sugimoto and Tohru Oki and Shigeko Murakami and Yukio Okano and Yoshinori Nozawa",

note = "Funding Information: ilckno~vlcr~~ottcWnlCs :t hank Rem! Wadhwn for crilial rending of the manuscript. This work was supported by the Ministry of Educn-(ion, Science and Cuhurc of Japan (Y.N.) and tlrc aycncy of Sclcncc and Technology (Y S.).",

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doi = "10.1016/0014-5793(91)80407-T",

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pages = "263--266",

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T1 - Calcium oscillation associated with reduced protein kinase C activities in ras-transformed NIH3T3 cells

AU - Fu, Tao

AU - Sugimoto, Yoshikazu

AU - Oki, Tohru

AU - Murakami, Shigeko

AU - Okano, Yukio

AU - Nozawa, Yoshinori

N1 - Funding Information: ilckno~vlcr~~ottcWnlCs :t hank Rem! Wadhwn for crilial rending of the manuscript. This work was supported by the Ministry of Educn-(ion, Science and Cuhurc of Japan (Y.N.) and tlrc aycncy of Sclcncc and Technology (Y S.).

PY - 1991/4/9

Y1 - 1991/4/9

N2 - We show here novel intracellular Ca2+ oscillation in ν-K-ras-transformed NIH3T3 cells induced by mitogenic peptide hormones, bradykinin and bombesin, as well as fetal calf serum. Induction of the Ca2+ oscillation is strongly correlated with the malignant properties and inversely with PKC activities in vitro and in vivo. These results suggest that the mitogen-induced Ca2+ oscillation is negatively regulated by PKC, which modulates Ca2+ influx in ν-K-ras-transformed NIH3T3 cells.

AB - We show here novel intracellular Ca2+ oscillation in ν-K-ras-transformed NIH3T3 cells induced by mitogenic peptide hormones, bradykinin and bombesin, as well as fetal calf serum. Induction of the Ca2+ oscillation is strongly correlated with the malignant properties and inversely with PKC activities in vitro and in vivo. These results suggest that the mitogen-induced Ca2+ oscillation is negatively regulated by PKC, which modulates Ca2+ influx in ν-K-ras-transformed NIH3T3 cells.

KW - Ca oscillation

KW - Protein kinase C

KW - ras-Transformed fibroblast

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Calcium oscillation associated with reduced protein kinase C activities in ras-transformed NIH3T3 cells

Abstract

Keywords

ASJC Scopus subject areas

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