Calcium release-activated calcium (CRAC) channels mediate the β2-adrenergic regulation of Na,K-ATPase

Michael J. Keller; Emilia Lecuona; Murali Prakriya; Yuan Cheng; Saul Soberanes; G. R.Scott Budinger; Jacob I. Sznajder

doi:10.1016/j.febslet.2014.10.041

Calcium release-activated calcium (CRAC) channels mediate the β₂-adrenergic regulation of Na,K-ATPase

Michael J. Keller, Emilia Lecuona^*, Murali Prakriya, Yuan Cheng, Saul Soberanes, G. R.Scott Budinger, Jacob I. Sznajder

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

β₂-Adrenergic agonists have been shown to regulate Na,K-ATPase in the alveolar epithelium by recruiting Na,K-ATPase-containing vesicles to the plasma membrane of alveolar epithelial cells (AEC). Here, we provide evidence that β₂-agonists induce store-operated calcium entry (SOCE) in AECs. This calcium entry is necessary for β₂-agonist-induced recruitment of Na,K-ATPase to the plasma membrane of AECs. Specifically, we show that β₂-agonists induce SOCE via stromal interaction molecule 1 (STIM1)-associated calcium release-activated calcium (CRAC) channels. We also demonstrate that the magnitude of SOCE affects the abundance of Na,K-ATPase at the plasma membrane of AECs.

Original language	English (US)
Pages (from-to)	4686-4693
Number of pages	8
Journal	FEBS Letters
Volume	588
Issue number	24
DOIs	https://doi.org/10.1016/j.febslet.2014.10.041
State	Published - Dec 20 2014

Keywords

Calcium channel
Calcium signaling
Epithelial cell
Na,K-ATPase
Store-operated calcium entry

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/j.febslet.2014.10.041

Cite this

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title = "Calcium release-activated calcium (CRAC) channels mediate the β2-adrenergic regulation of Na,K-ATPase",

abstract = "β2-Adrenergic agonists have been shown to regulate Na,K-ATPase in the alveolar epithelium by recruiting Na,K-ATPase-containing vesicles to the plasma membrane of alveolar epithelial cells (AEC). Here, we provide evidence that β2-agonists induce store-operated calcium entry (SOCE) in AECs. This calcium entry is necessary for β2-agonist-induced recruitment of Na,K-ATPase to the plasma membrane of AECs. Specifically, we show that β2-agonists induce SOCE via stromal interaction molecule 1 (STIM1)-associated calcium release-activated calcium (CRAC) channels. We also demonstrate that the magnitude of SOCE affects the abundance of Na,K-ATPase at the plasma membrane of AECs.",

keywords = "Calcium channel, Calcium signaling, Epithelial cell, Na,K-ATPase, Store-operated calcium entry",

author = "Keller, {Michael J.} and Emilia Lecuona and Murali Prakriya and Yuan Cheng and Saul Soberanes and Budinger, {G. R.Scott} and Sznajder, {Jacob I.}",

note = "Funding Information: We would like to thank Lynn Welch, BS for assistance in the manuscript preparation as well as Aparna Sundaram, MD and Nimrod Deiss-Yehiely, BS for technical support. This study was funded by HL48129, HL-76139, and HL-71643. Publisher Copyright: {\textcopyright} 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.",

year = "2014",

month = dec,

day = "20",

doi = "10.1016/j.febslet.2014.10.041",

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pages = "4686--4693",

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T1 - Calcium release-activated calcium (CRAC) channels mediate the β2-adrenergic regulation of Na,K-ATPase

AU - Keller, Michael J.

AU - Lecuona, Emilia

AU - Prakriya, Murali

AU - Cheng, Yuan

AU - Soberanes, Saul

AU - Budinger, G. R.Scott

AU - Sznajder, Jacob I.

N1 - Funding Information: We would like to thank Lynn Welch, BS for assistance in the manuscript preparation as well as Aparna Sundaram, MD and Nimrod Deiss-Yehiely, BS for technical support. This study was funded by HL48129, HL-76139, and HL-71643. Publisher Copyright: © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

PY - 2014/12/20

Y1 - 2014/12/20

N2 - β2-Adrenergic agonists have been shown to regulate Na,K-ATPase in the alveolar epithelium by recruiting Na,K-ATPase-containing vesicles to the plasma membrane of alveolar epithelial cells (AEC). Here, we provide evidence that β2-agonists induce store-operated calcium entry (SOCE) in AECs. This calcium entry is necessary for β2-agonist-induced recruitment of Na,K-ATPase to the plasma membrane of AECs. Specifically, we show that β2-agonists induce SOCE via stromal interaction molecule 1 (STIM1)-associated calcium release-activated calcium (CRAC) channels. We also demonstrate that the magnitude of SOCE affects the abundance of Na,K-ATPase at the plasma membrane of AECs.

AB - β2-Adrenergic agonists have been shown to regulate Na,K-ATPase in the alveolar epithelium by recruiting Na,K-ATPase-containing vesicles to the plasma membrane of alveolar epithelial cells (AEC). Here, we provide evidence that β2-agonists induce store-operated calcium entry (SOCE) in AECs. This calcium entry is necessary for β2-agonist-induced recruitment of Na,K-ATPase to the plasma membrane of AECs. Specifically, we show that β2-agonists induce SOCE via stromal interaction molecule 1 (STIM1)-associated calcium release-activated calcium (CRAC) channels. We also demonstrate that the magnitude of SOCE affects the abundance of Na,K-ATPase at the plasma membrane of AECs.

KW - Calcium channel

KW - Calcium signaling

KW - Epithelial cell

KW - Na,K-ATPase

KW - Store-operated calcium entry

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