Calcium signaling pathways utilized by P2X receptors in freshly isolated preglomerular MVSMC

Steven M. White, John D. Imig, Thu Thuy Kim, Benjamin C. Hauschild, Edward W. Inscho*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

40 Scopus citations


This study tested the hypothesis that P2X receptor activation increases intracellular Ca2+ concentration ([Ca2+]i) in preglomerular microvascular smooth muscle cells (MVSMC) by evoking voltage-dependent calcium influx. MVSMC were obtained and loaded with the calcium-sensitive dye fura 2 and studied by using single-cell fluorescence microscopy. The effect of P2X receptor activation on [Ca2+]i was assessed by using the P2X receptor-selective agonist α,β-methylene-ATP and was compared with responses elicited by the endogenous P2 receptor agonist ATP. α,β-Methylene-ATP increased [Ca2+]i dose dependently. Peak increases in [Ca2+]i averaged 37 ± 11, 73 ± 15, and 103 ± 21 nM at agonist concentrations of 0.1, 1, and 10 μM, respectively. The average peak response elicited by 10 μM α,β-methylene-ATP was ∼34% of the response obtained with 10 μM ATP. α,β-Methylene-ATP induced a transient increase in [Ca2+]i before [Ca2+]i returned to baseline, whereas ATP induced a biphasic response including a peak response followed by a sustained plateau. In Ca2+-free medium, ATP induced a sharp transient increase in [Ca2+]i, whereas the response to α,β-methylene-ATP was abolished. Ca2+ channel blockade with 10 μM diltiazem or nifedipine attenuated the response to α,β-methylene-ATP, whereas nonspecific blockade of Ca2+ influx pathways with 5 mM Ni2+ abolished the response. Blockade of P2X receptors with the novel P2X receptor antagonist NF-279 completely but reversibly abolished the response to α,β-methylene-ATP. These results indicate that P2X receptor activation by α,β-methylene-ATP increases [Ca2+]i in preglomerular MVSMC, in part, by stimulating voltage-dependent Ca2+ influx through L-type Ca2+ channels.

Original languageEnglish (US)
Pages (from-to)F1054-F1061
JournalAmerican Journal of Physiology - Renal Physiology
Issue number6 49-6
StatePublished - 2001


  • 8,8′-[carbonylbis (imino-4,1-phenylenecarbonylimino-4,1-phenylene-carbonylimino)]bis-1,3,5- napthalenetrisulfonic acid hexasodium salt
  • Adeonsine 5′-triphosphate
  • Afferent arteriole
  • Calcium channels
  • Diltiazem
  • Microvascular smooth muscle cells
  • Nickel
  • Nifedipine
  • P2X receptors
  • renal microvasculature
  • α,β-methylene-adeonsine 5′-triphosphate

ASJC Scopus subject areas

  • Physiology
  • Urology


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