Calibration of clot-based, coagulation factor assays using a first-order exponential equation and the method of nonlinear least squares

Gregory S. Retzinger*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

A nonlinear mathematical model has been developed which relates the time to calcium-initiated formation of a detectable clot in citrated, factor-deficient plasma to the clotting activity of the factor deficient in the plasma. The data of clot-based factor assays fit well the exponential expression of this scheme, which can be used conveniently in the clinical laboratory to calibrate the clotting activity of individual coagulation factors in samples of citrated plasma. Aside from its clinical utility, the model may prompt the formulation of testable hypotheses not otherwise prompted by the existing, empiric approach to the quantitation of coagulation factor activity.

Original languageEnglish (US)
Pages (from-to)139-148
Number of pages10
JournalHaematologia
Volume28
Issue number3
StatePublished - Dec 1 1997

Keywords

  • Clinical coagulation
  • Coagulation factor activity
  • Factor assay calibration
  • Nonlinear least squares analysis

ASJC Scopus subject areas

  • Hematology

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