cAMP-activated chloride channels in a MR-transfected pancreatic adenocarcinoma-derived cell line, pANS6

Annabel N. Smith, Catherine J C Wardle, John P. Winpenny, Bernard Verdon, Michael A. Gray, Barry E. Argent, Ann Harris*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Pancreatic adenocarcinoma cell lines rarely express the CFTR gene, despite the high levels of CFTR protein that are present in primary pancreatic duct cells. We have attempted to generate a non-CF pancreatic adenocarcinoma cell line that stably produces high levels of CFTR mRNA and protein by transfecting a vector containing the CFTR cDNA, driven by a strong mammalian promoter, into the poorly differentiated pancreatic adenocarcinoma cell line, Panc-1. The pANS6 pancreatic duct cell line expresses substantial levels of CFTR mRNA, but little CFTR protein. Despite this we were able to detect low conductance chloride channels in 40% of patches, stimulated with cAMP, that have similar biophysical properties to CFTR.

Original languageEnglish (US)
Pages (from-to)315-320
Number of pages6
JournalBBA - Molecular Basis of Disease
Volume1271
Issue number2-3
DOIs
StatePublished - Jun 9 1995

Keywords

  • Adenocarcinoma, pancreatic
  • CFTR, cystic fibrosis transmembrane regulator
  • Chloride channel
  • cAMP activation

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology

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