Cancer-cell-secreted CXCL11 promoted CD8 + T cells infiltration through docetaxel-induced-release of HMGB1 in NSCLC

Qun Gao, Shumin Wang, Xinfeng Chen, Shaoyan Cheng, Zhen Zhang, Feng Li, Lan Huang, Yang Yang, Bin Zhou, Dongli Yue, Dan Wang, Ling Cao, Nomathamsanqa Resegofetse Maimela, Bin Zhang, Jane Yu, Liping Wang*, Yi Zhang

*Corresponding author for this work

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: Chemotherapy combined with immunotherapy becomes the main trend in lung cancer intervention; however, how chemotherapy promotes the immune function remains elusive. Therefore, we sought to determine how chemotherapy promotes the immune function. Methods: We determined in 100 NSCLC patients the expression of CD8, functional markers (IFN-γ, Granzyme B, and Perforin) and specific chemokines by quantitative real-time reverse transcriptase-PCR. Functional experiments were carried out to check whether docetaxel (DOC), a chemotherapeutic agent, modifies the expression of HMGB1 and CXCL11, and influences the infiltration properties of CD8 + T cells to the tumor microenvironment. The mechanism of the release of HMGB1 and CXCL11 was determined by flow cytometry, immunofluorescence and western blotting. In in vivo experiment, we confirmed how DOC enhanced the recruitment of HER2-CAR T cells to tumor sites. Results: We found that DOC upregulated the expression of chemokine receptor ligand CXCL11 in tumor microenvironment and subsequently enhanced CD8 + T cell recruitment. DOC treatment significantly increased HMGB1 release in an ROS-dependent manner. Recombinant protein HMGB1 stimulated the secretion of CXCL11 via NF-κB activation in vitro. Tumors from DOC-treated mice exhibited higher expression of HMGB1 and CXCL11, more HER2-CAR T cell infiltration, and reduced progression, relative to control. Increased HMGB1 and CXCL11 expressions were positively correlated with prolonged overall survival of lung cancer patients. Conclusions: Our results demonstrate that DOC induces CD8 + T cell recruitment to the tumor microenvironment by enhancing the secretion of HMGB1 and CXCL11, thus improving the anti-tumor efficacy, indicating that modulating the HMGB1-CXCL11 axis might be helpful for NSCLC treatment.

Original languageEnglish (US)
Article number42
JournalJournal for ImmunoTherapy of Cancer
Volume7
Issue number1
DOIs
StatePublished - Feb 11 2019

Fingerprint

docetaxel
HMGB1 Protein
T-Lymphocytes
Tumor Microenvironment
Neoplasms
Drug Therapy
Lung Neoplasms
Chemokine Receptors
Reverse Transcriptase Polymerase Chain Reaction
Chemokines
Recombinant Proteins
Immunotherapy
Fluorescent Antibody Technique

Keywords

  • CD8 T cells
  • CXCL11
  • Docetaxel
  • HER2-CAR T cells; high-mobility group box-1
  • Non-small cell lung cancer

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Molecular Medicine
  • Oncology
  • Pharmacology
  • Cancer Research

Cite this

Gao, Qun ; Wang, Shumin ; Chen, Xinfeng ; Cheng, Shaoyan ; Zhang, Zhen ; Li, Feng ; Huang, Lan ; Yang, Yang ; Zhou, Bin ; Yue, Dongli ; Wang, Dan ; Cao, Ling ; Maimela, Nomathamsanqa Resegofetse ; Zhang, Bin ; Yu, Jane ; Wang, Liping ; Zhang, Yi. / Cancer-cell-secreted CXCL11 promoted CD8 + T cells infiltration through docetaxel-induced-release of HMGB1 in NSCLC In: Journal for ImmunoTherapy of Cancer. 2019 ; Vol. 7, No. 1.
@article{8c6e5b9763224f498b1714a34d3b139e,
title = "Cancer-cell-secreted CXCL11 promoted CD8 + T cells infiltration through docetaxel-induced-release of HMGB1 in NSCLC",
abstract = "Background: Chemotherapy combined with immunotherapy becomes the main trend in lung cancer intervention; however, how chemotherapy promotes the immune function remains elusive. Therefore, we sought to determine how chemotherapy promotes the immune function. Methods: We determined in 100 NSCLC patients the expression of CD8, functional markers (IFN-γ, Granzyme B, and Perforin) and specific chemokines by quantitative real-time reverse transcriptase-PCR. Functional experiments were carried out to check whether docetaxel (DOC), a chemotherapeutic agent, modifies the expression of HMGB1 and CXCL11, and influences the infiltration properties of CD8 + T cells to the tumor microenvironment. The mechanism of the release of HMGB1 and CXCL11 was determined by flow cytometry, immunofluorescence and western blotting. In in vivo experiment, we confirmed how DOC enhanced the recruitment of HER2-CAR T cells to tumor sites. Results: We found that DOC upregulated the expression of chemokine receptor ligand CXCL11 in tumor microenvironment and subsequently enhanced CD8 + T cell recruitment. DOC treatment significantly increased HMGB1 release in an ROS-dependent manner. Recombinant protein HMGB1 stimulated the secretion of CXCL11 via NF-κB activation in vitro. Tumors from DOC-treated mice exhibited higher expression of HMGB1 and CXCL11, more HER2-CAR T cell infiltration, and reduced progression, relative to control. Increased HMGB1 and CXCL11 expressions were positively correlated with prolonged overall survival of lung cancer patients. Conclusions: Our results demonstrate that DOC induces CD8 + T cell recruitment to the tumor microenvironment by enhancing the secretion of HMGB1 and CXCL11, thus improving the anti-tumor efficacy, indicating that modulating the HMGB1-CXCL11 axis might be helpful for NSCLC treatment.",
keywords = "CD8 T cells, CXCL11, Docetaxel, HER2-CAR T cells; high-mobility group box-1, Non-small cell lung cancer",
author = "Qun Gao and Shumin Wang and Xinfeng Chen and Shaoyan Cheng and Zhen Zhang and Feng Li and Lan Huang and Yang Yang and Bin Zhou and Dongli Yue and Dan Wang and Ling Cao and Maimela, {Nomathamsanqa Resegofetse} and Bin Zhang and Jane Yu and Liping Wang and Yi Zhang",
year = "2019",
month = "2",
day = "11",
doi = "10.1186/s40425-019-0511-6",
language = "English (US)",
volume = "7",
journal = "Journal for ImmunoTherapy of Cancer",
issn = "2051-1426",
publisher = "BioMed Central",
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Gao, Q, Wang, S, Chen, X, Cheng, S, Zhang, Z, Li, F, Huang, L, Yang, Y, Zhou, B, Yue, D, Wang, D, Cao, L, Maimela, NR, Zhang, B, Yu, J, Wang, L & Zhang, Y 2019, ' Cancer-cell-secreted CXCL11 promoted CD8 + T cells infiltration through docetaxel-induced-release of HMGB1 in NSCLC ', Journal for ImmunoTherapy of Cancer, vol. 7, no. 1, 42. https://doi.org/10.1186/s40425-019-0511-6

Cancer-cell-secreted CXCL11 promoted CD8 + T cells infiltration through docetaxel-induced-release of HMGB1 in NSCLC . / Gao, Qun; Wang, Shumin; Chen, Xinfeng; Cheng, Shaoyan; Zhang, Zhen; Li, Feng; Huang, Lan; Yang, Yang; Zhou, Bin; Yue, Dongli; Wang, Dan; Cao, Ling; Maimela, Nomathamsanqa Resegofetse; Zhang, Bin; Yu, Jane; Wang, Liping; Zhang, Yi.

In: Journal for ImmunoTherapy of Cancer, Vol. 7, No. 1, 42, 11.02.2019.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Cancer-cell-secreted CXCL11 promoted CD8 + T cells infiltration through docetaxel-induced-release of HMGB1 in NSCLC

AU - Gao, Qun

AU - Wang, Shumin

AU - Chen, Xinfeng

AU - Cheng, Shaoyan

AU - Zhang, Zhen

AU - Li, Feng

AU - Huang, Lan

AU - Yang, Yang

AU - Zhou, Bin

AU - Yue, Dongli

AU - Wang, Dan

AU - Cao, Ling

AU - Maimela, Nomathamsanqa Resegofetse

AU - Zhang, Bin

AU - Yu, Jane

AU - Wang, Liping

AU - Zhang, Yi

PY - 2019/2/11

Y1 - 2019/2/11

N2 - Background: Chemotherapy combined with immunotherapy becomes the main trend in lung cancer intervention; however, how chemotherapy promotes the immune function remains elusive. Therefore, we sought to determine how chemotherapy promotes the immune function. Methods: We determined in 100 NSCLC patients the expression of CD8, functional markers (IFN-γ, Granzyme B, and Perforin) and specific chemokines by quantitative real-time reverse transcriptase-PCR. Functional experiments were carried out to check whether docetaxel (DOC), a chemotherapeutic agent, modifies the expression of HMGB1 and CXCL11, and influences the infiltration properties of CD8 + T cells to the tumor microenvironment. The mechanism of the release of HMGB1 and CXCL11 was determined by flow cytometry, immunofluorescence and western blotting. In in vivo experiment, we confirmed how DOC enhanced the recruitment of HER2-CAR T cells to tumor sites. Results: We found that DOC upregulated the expression of chemokine receptor ligand CXCL11 in tumor microenvironment and subsequently enhanced CD8 + T cell recruitment. DOC treatment significantly increased HMGB1 release in an ROS-dependent manner. Recombinant protein HMGB1 stimulated the secretion of CXCL11 via NF-κB activation in vitro. Tumors from DOC-treated mice exhibited higher expression of HMGB1 and CXCL11, more HER2-CAR T cell infiltration, and reduced progression, relative to control. Increased HMGB1 and CXCL11 expressions were positively correlated with prolonged overall survival of lung cancer patients. Conclusions: Our results demonstrate that DOC induces CD8 + T cell recruitment to the tumor microenvironment by enhancing the secretion of HMGB1 and CXCL11, thus improving the anti-tumor efficacy, indicating that modulating the HMGB1-CXCL11 axis might be helpful for NSCLC treatment.

AB - Background: Chemotherapy combined with immunotherapy becomes the main trend in lung cancer intervention; however, how chemotherapy promotes the immune function remains elusive. Therefore, we sought to determine how chemotherapy promotes the immune function. Methods: We determined in 100 NSCLC patients the expression of CD8, functional markers (IFN-γ, Granzyme B, and Perforin) and specific chemokines by quantitative real-time reverse transcriptase-PCR. Functional experiments were carried out to check whether docetaxel (DOC), a chemotherapeutic agent, modifies the expression of HMGB1 and CXCL11, and influences the infiltration properties of CD8 + T cells to the tumor microenvironment. The mechanism of the release of HMGB1 and CXCL11 was determined by flow cytometry, immunofluorescence and western blotting. In in vivo experiment, we confirmed how DOC enhanced the recruitment of HER2-CAR T cells to tumor sites. Results: We found that DOC upregulated the expression of chemokine receptor ligand CXCL11 in tumor microenvironment and subsequently enhanced CD8 + T cell recruitment. DOC treatment significantly increased HMGB1 release in an ROS-dependent manner. Recombinant protein HMGB1 stimulated the secretion of CXCL11 via NF-κB activation in vitro. Tumors from DOC-treated mice exhibited higher expression of HMGB1 and CXCL11, more HER2-CAR T cell infiltration, and reduced progression, relative to control. Increased HMGB1 and CXCL11 expressions were positively correlated with prolonged overall survival of lung cancer patients. Conclusions: Our results demonstrate that DOC induces CD8 + T cell recruitment to the tumor microenvironment by enhancing the secretion of HMGB1 and CXCL11, thus improving the anti-tumor efficacy, indicating that modulating the HMGB1-CXCL11 axis might be helpful for NSCLC treatment.

KW - CD8 T cells

KW - CXCL11

KW - Docetaxel

KW - HER2-CAR T cells; high-mobility group box-1

KW - Non-small cell lung cancer

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U2 - 10.1186/s40425-019-0511-6

DO - 10.1186/s40425-019-0511-6

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JO - Journal for ImmunoTherapy of Cancer

JF - Journal for ImmunoTherapy of Cancer

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