Cancer/testis antigen HCA587-derived long peptide vaccine generates potent immunologic responses and antitumor effects in mouse model

Lijie Zhang, Juanjuan Chen, Xiao Song, Weigang Wen, Yan Li, Yu Zhang, Yanhui Yin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The cunccr/testis antigen HCA587 (also known as MAGE-C2), one of the most immunogenic tumor antigens, is overexpressed in a wide spectrum of malignant tumors and can serve as a target for immunotherapy. In this study, we synthesized 14 overlapping (25-35 amino acids) long peptides representing the sequence of the most immunogenic part of the HCA587 protein and evaluated the antigen-specific immune responses and antitumor effects generated by immunization with the synthetic long peptide (SLP) vaccine in a mouse model. HCA587 SLPs in combination with adjuvants CFA and CpG ODN induced potent T-cell responses, which were dominated by type I cytokine IFN-γ-producing CD4+ T cells as measured by ELISPOT and intracellular cytokine staining assay. Moreover, HCA587 SLP vaccination conferred protection against challenge with HCA587-expressing B16 melanoma in a therapeutic setting. Our findings may provide a scientific basis for the use of HCA587-derived long overlapping peptide vaccine for the treatment of patients with cancer in future clinical trials.

Original languageEnglish (US)
Pages (from-to)193-200
Number of pages8
JournalOncology Research
Volume21
Issue number4
DOIs
StatePublished - 2014

Keywords

  • Cancer/testis antigen
  • HCA587 (MAGE-C2)
  • Synthetic long peptide (SLP)
  • Tumor
  • Vaccine

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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